Identification of Vibrio parahaemolyticus based on the concatenated DNA sequencing of 16S rRNA and gyrB gene
10.19428/j.cnki.sjpm.2021.20874
- VernacularTitle:基于16S rRNA和gyrB基因串联DNA特征序列的副溶血性弧菌鉴定
- Author:
Zhen FENG
1
;
Zhi-hua ZHANG
1
;
Shan-shan XIAO
1
;
Huan ZHANG
1
;
Feng QIN
1
;
Mei-cheng YANG
1
Author Information
1. Shanghai Institute for Food and Drug Control， NMPA Key Laboratory for Testing Technology ofPharmaceutical Microbiology， Shanghai 201203， China
- Publication Type:Research Article
- Keywords:
Vibrio parahaemolyticus;
16S rRNA gene;
gyrB gene;
concatenated nucleotide sequence;
phylogenetic analysis
- From:
Shanghai Journal of Preventive Medicine
2021;33(5):372-376
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish the concatenated DNA sequencing of 16S ribosomal RNA （16S rRNA） and DNA gyrase subunit B （gyrB） gene， and provide evidence for the identification and classification of Vibrio parahaemolyticus （V. parahaemolyticus）. Methods:Typical strains in the genus of Vibrio spp. was selected， such as V. parahaemolyticus， V. alginolyticus and other species for examination of 16S rRNA and gyrB gene as target. Primers were separately designed to amplify these two nucleotide fragments. Phylogenetic analysis was performed using the concatenated sequences. Results:The concatenated 16S rRNA+gyrB nucleotide sequence of V. parahaemolyticus formed a single cluster in the phylogenetic analysis， which identified the typical strains of Vibro spp. at the species level. Conclusion:In our study， an identification method of V. parahaemolyticus is established based on concatenated 16S rRNA+gyrB nucleotide sequencing. It can identify the strains of V. parahaemolyticus at the species level， which may be applied in phylogenetic analysis and contamination tracing of V. parahaemolyticus in food and drug control.
