Content Determination of 6 Kinds of Monosaccharide in Astragalus membranaceus with Different Growth Years by Pre-column Derivatization-HPLC
- VernacularTitle:PMP柱前衍生化-HPLC法分析不同生长年限黄芪中6种单糖的含量
- Author:
Yiting YU
1
;
Wenxia PI
1
;
Hui XIE
1
;
Lijuan CAO
2
;
Xiwen LI
3
;
Xia LI
4
;
Tulin LU
1
;
Guojun YAN
1
Author Information
1. School of Pharmacy，Nanjing University of TCM，Nanjing 210023，China
2. Shengshi Baicao Pharmaceutical Co.，Ltd.，Tianjin 300301，China
3. Institute of Chinese Materia Medica，China Academy of Chinese Medical Sciences，Beijing 100700，China
4. School of Pharmaceutical Science and Technology，Tianjin University，Tianjin 300072，China
- Publication Type:Journal Article
- Keywords:
Astragalus membra naceus;
Polysaccharides;
Monosaccharides;
Pre-column derivatization;
HPLC
- From:
China Pharmacy
2021;32(12):1448-1452
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE：To analyze and compare th e contents of 6 kinds of monosaccharide in Astragalus membranaceus from different growth years . METHODS ：2-4 years old A. membranaceus from three areas were extracted with water extraction and alcohol precipitation ，Sevage deproteinization to obtain A. membranaceus polysaccharide. The samples were firstly hydrolyzed with trifluoroacetic acid （TFA）and then derivatized by 1-phenyl-3-methyl-5-pyrazolone（PMP）. HPLC analysis was adopted to determine the contents of 6 kinds of monosaccharide as mannose ，rhamnose，galacturonic acid ，glucose，galactose，arabinose. The determination was performed on Symmetry C 18 column with phosphate buffer solution （pH 6.8）-acetonitrile（84∶16，V/V）as mobile phase at the flow rate of 1.0 mL/min. The detection wavelength was 245 nm，and column temperature was 35 ℃. The sample size was 20 µL. RESULTS ：The contents of mannose ，rhamnose，galacturonic acid ，glucose，galactose and arabinose were 0.50-0.94， 0.76-1.60，3.35-7.86，87.33-275.77，1.95-8.96，2.35-14.04 mg/g，respectively. Total contents of monosaccharide from 2，3，4 years old A. membranaceus were 98.26-139.92，173.81-295.71，122.37-182.41 mg/g，respectively. There was significant difference in the contents of glucose between 3 old years A. membranaceus and 2，4 old years A. membranaceus （162.71-275.77 mg/g vs. 87.33-107.70，111.54-167.26 mg/g，P＜0.05）. CONCLUSIONS ：Above 6 monosaccharides are detected in 2，3，4 years old A. membranaceus，among which the content of glucose is the highest. The content of glucose in 3 years old A. membranaceus is higher than that in 2 and 4 years old A. membranaceus .
