SMYD3-PARP16 axis accelerates unfolded protein response and mediates neointima formation.
10.1016/j.apsb.2020.12.010
- Author:
Fen LONG
1
;
Di YANG
1
;
Jinghua WANG
1
;
Qing WANG
1
;
Ting NI
2
;
Gang WEI
2
;
Yizhun ZHU
3
;
Xinhua LIU
1
Author Information
1. Pharmacophenomics Laboratory, Human Phenome Institute, Fudan University, Shanghai 201203, China.
2. State Key Laboratory of Genetic Engineering & MOE Key Laboratory of Contemporary Anthropology, Collaborative Innovation Center of Genetics and Development, School of Life Sciences and Shanghai Cancer Center, Fudan University, Shanghai 200438, China.
3. State Key Laboratory of Quality Research in Chinese Medicine and School of Pharmacy, Macau University of Science and Technology, Macau, China.
- Publication Type:Journal Article
- Keywords:
ATF6, activating transcription factor 6;
BIP, immunoglobulin heavy-chain binding protein;
ChIP-seq, chromatin immunoprecipitation coupled with deep sequencing;
DAPI, 4′,6-diamidino-2-phenylindole;
ECM, extracellular matrix;
EGCG, epigallocatechin-3-gallate;
ER, endoplasmic reticulum;
Endoplasmic reticulum;
H3K4, histone H3 lysine 4;
IACUC, Institutional Animal Care and Use Committee;
IRE1, inositol-requiring enzyme 1;
MMP, matrix metal proteinase;
Neointimal hyperplasia;
PARP, poly(ADP-ribose) polymerases;
PARP16;
PCNA, proliferating cell nuclear antigen;
PDGF, platelet-derived growth factor;
PERK, protein kinase R (PKR)-like ER kinase;
SMCs, smooth muscle cells;
SMYD3;
SMYD3, SET and MYND domain containing 3;
UPR, unfolded protein response;
VCAM-1, vascular cell adhesion molecule-1;
VSMCs, vascular smooth muscle cells;
Vascular smooth muscle cell;
XBP-1, X-box binding protein-1;
p-PERK, phosphate-PKR-like ER kinase;
p-eIF2α, phosphate-eukaryotic initiation factor 2α;
siRNA, small interfering RNA
- From:
Acta Pharmaceutica Sinica B
2021;11(5):1261-1273
- CountryChina
- Language:English
-
Abstract:
Neointimal hyperplasia after vascular injury is a representative complication of restenosis. Endoplasmic reticulum (ER) stress-induced unfolded protein response (UPR) is involved in the pathogenesis of vascular intimal hyperplasia. PARP16, a member of the poly(ADP-ribose) polymerases family, is correlated with the nuclear envelope and the ER. Here, we found that PERK and IRE1
