A four-protein metabolon assembled by a small peptide protein creates the pentacyclic carbonate ring of aldgamycins.

Author: Qiaozhen WANG ¹ ; Xiaolong TANG ² ; Ping DAI ¹ ; Chuanxi WANG ¹ ; Weiyang ZHANG ² ; Guodong CHEN ¹ ; Kui HONG ³ ; Dan HU ¹ ; Hao GAO ¹ ; Xinsheng YAO ¹
Author Information

1. Institute of Traditional Chinese Medicine and Natural Product/Guangdong Province Key Laboratory of Pharmacodynamic Constituents of TCM and New Drugs Research, College of Pharmacy, Jinan University, Guangzhou 510632, China.
2. Institute of Synthetic Biology, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China.
3. Key Laboratory of Combinatorial Biosynthesis and Drug Discovery, Ministry of Education, School of Pharmaceutical Sciences, Wuhan University, Wuhan 430072, China.
Publication Type:Journal Article
Keywords: Aldgamycins; Biosynthesis; N-hydroxylcarbamoyl; Organic carbonate; Protein complex
From: Acta Pharmaceutica Sinica B 2021;11(2):588-597
CountryChina
Language:English
Abstract: Organic carbonates (OCs) are a class of compounds featured by a carbonyl flanked by two alkoxy/aryloxy groups. They exist in either linear or cyclic forms, of which the majority encountered in nature adopt a pentacyclic structure. However, the enzymatic basis for pentacyclic carbonate ring formation remains elusive. Here, we reported that a four-protein metabolon (AlmUII-UV) assembled by a small peptide protein (AlmUV) appends a reactive