Comprehensive profiling and characterization of the absorbed components and metabolites in mice serum and tissues following oral administration of Qing-Fei-Pai-Du decoction by UHPLC-Q-Exactive-Orbitrap HRMS.
10.1016/S1875-5364(21)60031-6
- Author:
Wei LIU
1
;
Jian HUANG
2
;
Feng ZHANG
2
;
Cong-Cong ZHANG
3
,
4
;
Rong-Sheng LI
1
;
Yong-Li WANG
5
;
Chao-Ran WANG
6
;
Xin-Miao LIANG
6
;
Wei-Dong ZHANG
2
;
Ling YANG
2
;
Ping LIU
3
,
7
;
Guang-Bo GE
8
Author Information
1. Key Laboratory of Liver and Kidney Diseases (Ministry of Education), Institute of Liver Diseases, Shanghai Key Laboratory of Traditional Chinese Clinical Medicine, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.
2. Institute of Interdisciplinary Integrative Medicine Research, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.
3. Key Laboratory of Liver and Kidney Diseases (Ministry of Education), Institute of Liver Diseases, Shanghai Key Laboratory of Traditional Chinese Clinical Medicine, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China
4. Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.
5. Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.
6. Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China.
7. Institute of Interdisciplinary Integrative Medicine Research, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China. Electronic address: liuliver@vip.sina.com.
8. Institute of Interdisciplinary Integrative Medicine Research, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China. Electronic address: geguangbo@dicp.ac.cn.
- Publication Type:Journal Article
- Keywords:
Absorbed components;
Chemical profiling;
Corona virus disease 2019 (COVID-19);
High-Resolution Mass Spectrometry (HRMS);
Qing-Fei-Pai-Du decoction (QFPDD)
- MeSH:
Administration, Oral;
Alkaloids/analysis*;
Animals;
COVID-19;
Chromatography, High Pressure Liquid;
Drugs, Chinese Herbal/pharmacokinetics*;
Flavonoids/analysis*;
Mice;
SARS-CoV-2;
Saponins/analysis*;
Triterpenes/analysis*
- From:
Chinese Journal of Natural Medicines (English Ed.)
2021;19(4):305-320
- CountryChina
- Language:English
-
Abstract:
Qing-Fei-Pai-Du decoction (QFPDD) is a Chinese medicine compound formula recommended for combating corona virus disease 2019 (COVID-19) by National Health Commission of the People's Republic of China. The latest clinical study showed that early treatment with QFPDD was associated with favorable outcomes for patient recovery, viral shedding, hospital stay, and course of the disease. However, the effective constituents of QFPDD remain unclear. In this study, an UHPLC-Q-Orbitrap HRMS based method was developed to identify the chemical constituents in QFPDD and the absorbed prototypes as well as the metabolites in mice serum and tissues following oral administration of QFPDD. A total of 405 chemicals, including 40 kinds of alkaloids, 162 kinds of flavonoids, 44 kinds of organic acids, 71 kinds of triterpene saponins and 88 kinds of other compounds in the water extract of QFPDD were tentatively identified via comparison with the retention times and MS/MS spectra of the standards or refereed by literature. With the help of the standards and in vitro metabolites, 195 chemical components (including 104 prototypes and 91 metabolites) were identified in mice serum after oral administration of QFPDD. In addition, 165, 177, 112, 120, 44, 53 constituents were identified in the lung, liver, heart, kidney, brain, and spleen of QFPDD-treated mice, respectively. These findings provided key information and guidance for further investigation on the pharmacologically active substances and clinical applications of QFPDD.