Regulation of RDN on Th1/ILC1 cell imbalance in HFMD patients caused by EV71 infection.
10.1016/S1875-5364(21)60022-5
- Author:
Yi-Meng WANG
1
;
Ye TIAN
2
;
Qian-Wen LI
1
;
Zheng-Ying BIAN
1
;
Yue GAO
1
;
Yu ZEN
1
;
Lei TANG
1
;
Tie-Jun TANG
1
;
Wei GUO
3
;
Wen-Bing YAO
4
Author Information
1. Jiangsu Key Laboratory of Druggability of Biopharmaceuticals, School of Life Science and Technology, China Pharmaceutical University, Nanjing 210009, China.
2. Children's Hospital of Nanjing Medical University, Nanjing 210009, China.
3. Jiangsu Key Laboratory of Druggability of Biopharmaceuticals, School of Life Science and Technology, China Pharmaceutical University, Nanjing 210009, China. Electronic address: guowei1205@cpu.edu.cn.
4. Jiangsu Key Laboratory of Druggability of Biopharmaceuticals, School of Life Science and Technology, China Pharmaceutical University, Nanjing 210009, China. Electronic address: wbyao@cpu.edu.cn.
- Publication Type:Journal Article
- Keywords:
Enterovirus 71 (EV71);
Hand, foot and mouth disease (HFMD);
Innate immunity;
Reduning injection (RDN);
Th1 differentiated in vitro
- From:
Chinese Journal of Natural Medicines (English Ed.)
2021;19(3):205-211
- CountryChina
- Language:English
-
Abstract:
Enterovirus 71 (EV71) infection is more likely to cause hand, foot and mouth disease (HFMD) in children, which can lead to neurogenic complications and higher mortality. As a commonly used clinical medicine, Reduning injection (RDN) helps to shorten the symptoms of patients with HFMD and facilitate the early recovery of children. However, the regulatory mechanism of RDN on the HFMD immune system disorder caused by EV71 remains to be discussed. This study collected detailed treatment data of 56 children with HFMD who entered the affiliated Children's Hospital of Nanjing Medical University during 2019. Retrospective analysis of clinical data showed that the symptoms of the RDN treatment group were improved compared with the untreated group. To explore its mechanism, the relevant detection indicators were detected by flow cytometry, enzyme-linked immunosorbent assay and real-time quantitative PCR. It was found that the number and function of innate immune (ILCs) and adaptive immunity (Th1, Th2 and secreted cytokines) were reduced, suggesting that RDN plays a role by regulating cellular immunity. The in vitro differentiation inhibition test further confirmed that RDN affected Th1 differentiation by inhibiting the expression of transcription factors on the basis of Th1 cell differentiation in vitro.
