cDNA cloning of a novel lectin that induce cell apoptosis from Artocarpus hypargyreus.
10.1016/S1875-5364(21)60009-2
- Author:
Yu LUO
1
;
Lin-Jie ZENG
2
;
Xiao-Qin LIU
1
;
Lu LI
1
;
Qi-Yan ZENG
3
Author Information
1. Department of Biochemistry and Molecular Biology, Guangxi Medical University, Nanning 530021, China.
2. Department of Orthopaedics, Chinese and Western Orthopaedics Hospital of Guigang, Guigang 537100, China.
3. Department of Biochemistry and Molecular Biology, Guangxi Medical University, Nanning 530021, China. Electronic address: zengnn6@hotmail.com.
- Publication Type:Journal Article
- Keywords:
Antitumor drug;
Apoptosis;
Artocarpus hypargyreus;
Lectin;
cDNA cloning
- From:
Chinese Journal of Natural Medicines (English Ed.)
2021;19(2):81-89
- CountryChina
- Language:English
-
Abstract:
We isolated a novel lectin (AHL) from Artocarpus hypargyreusHance and showed its immunomodulatory activities. In this study, the amino acid sequence of AHL was determined by cDNA sequencing. AHL cDNA (875bp) contains a 456-bp open reading frame (ORF), which encodes a protein with 151 amino acids. AHL is a new member of jacalin-related lectin family (JRLs), which share high sequence similarities to KM+ and Morniga M, and contain the conserved carbohydrate binding domains. The antitumor activity of AHL was also explored using Jurkat T cell lines. AHL exhibits a strong binding affinity to cell membrane, which can be effectively inhibited by methyl-α-D-galactose. AHL inhibits cell proliferation in a time- and dose-dependent manner through apoptosis, evidenced by morphological changes, phosphatidylserine externalization, poly ADP-ribose polymerase (PARP) cleavage, Bad and Bax up-regulation, and caspase-3 activation. We further showed that the activation of ERK and p38 signaling pathways is involved for the pro-apoptotic effect of AHL.
