A 75 kDa glycoprotein isolated from Cudrania tricuspidata Bureau induces colonic epithelial proliferation and ameliorates mouse colitis induced by dextran sulfate sodium.
10.1016/S1875-5364(21)60005-5
- Author:
Jeong-Bae PARK
1
;
Do-Wan KIM
1
;
Kye-Taek LIM
2
;
Sejong OH
3
;
Sei-Jung LEE
4
Author Information
1. Department of Pharmaceutical Engineering, Daegu Haany University, Gyeongsan 38610, South Korea.
2. Division of Animal Science, Chonnam National University, Gwangju 61186, South Korea.
3. Division of Animal Science, Chonnam National University, Gwangju 61186, South Korea. Electronic address: soh@jnu.ac.kr.
4. Department of Pharmaceutical Engineering, Daegu Haany University, Gyeongsan 38610, South Korea. Electronic address: sjlee@dhu.ac.kr.
- Publication Type:Journal Article
- Keywords:
Cell cycle;
Cell proliferation;
Colon epithelial cells;
Cudrania tricuspidata;
Glycoprotein;
Inflammatory bowel disease;
Protein kinase C
- From:
Chinese Journal of Natural Medicines (English Ed.)
2021;19(1):46-55
- CountryChina
- Language:English
-
Abstract:
Cudrania tricuspidata Bureau (CTB), a species of the Moraceae plant, has been used as a bruise recovery treatment. This study aimed to determine whether the 75 kDa phytoglycoprotein extracted from CTB has a regulatory effect on the proliferation of human colon epithelial cells and the pathological process of inflammatory bowel disease (IBD). We found that CTB glycoprotein significantly induces the proliferation of human colon epithelial HT-29 cells by activating protein kinase C. CTB glycoprotein stimulated the phosphorylation of c-Jun N-terminal kinase and transcription factor nuclear factor-κB, which are responsible for the expression of cell-cycle-related proteins (CDK2, CDK4, cyclin D1 and cyclin E) during its promotion of cell proliferation. Experimental colitis was induced in mice by adding dextran sulfate sodium to their drinking water at a concentration of 4% (W/V) for seven days. We found that CTB glycoprotein ameliorates the pathological process of IBD and lowers the disease activity index score, which was composed of body weight change, diarrhea, and hematochezia in ICR mice treated with dextran sulfate sodium. Hence, we suggest that CTB glycoprotein has the ability to prevent IBD by promoting cell proliferation signaling events via the activation of PKC, JNK and NF-κB in colon epithelial cells.
