Shunt products of aminoansamycins from aas1 overexpressed mutant strain of Streptomyces sp. S35.
10.1016/S1875-5364(20)60039-5
- Author:
Li-Ping DAI
1
;
Zi-Shen WANG
2
;
Hao-Xin WANG
2
;
Chun-Hua LU
3
;
Yue-Mao SHEN
4
,
5
Author Information
1. Key Laboratory of Chemical Biology (Ministry of Education), School of Pharmaceutical Sciences, Shandong University, Jinan 250012, China.
2. State Key Laboratory of Microbial Technology, Shandong University, Qingdao 266237, China.
3. Key Laboratory of Chemical Biology (Ministry of Education), School of Pharmaceutical Sciences, Shandong University, Jinan 250012, China. Electronic address: ahua0966@sdu.edu.cn.
4. Key Laboratory of Chemical Biology (Ministry of Education), School of Pharmaceutical Sciences, Shandong University, Jinan 250012, China
5. State Key Laboratory of Microbial Technology, Shandong University, Qingdao 266237, China. Electronic address: yshen@sdu.edu.cn.
- Publication Type:Journal Article
- Keywords:
Aminoansamycins;
Shunt products;
Streptomyces sp. S35
- MeSH:
Biological Products/metabolism*;
Lactams, Macrocyclic/metabolism*;
Multigene Family;
Organisms, Genetically Modified;
Streptomyces/metabolism*
- From:
Chinese Journal of Natural Medicines (English Ed.)
2020;18(12):952-956
- CountryChina
- Language:English
-
Abstract:
Constitutively expression of the pathway-specific activators is an effective method to activate silent gene clusters and improve natural product production. In this study, nine shunt products of aminoansamycins (1-9) were identified from a recombinant mutant strain S35-LAL by overexpressed the large-ATP-binding regulator of the LuxR family (LAL) gene aas1 in Streptomyces sp. S35. All the compounds showed no anti-microbial, anti-T3SS and cytotoxic activities.
