Ganoderic acid A protects lens epithelial cells from UVB irradiation and delays lens opacity.
10.1016/S1875-5364(20)60037-1
- Author:
Li-Hua KANG
1
;
Guo-Wei ZHANG
1
;
Jun-Fang ZHANG
1
;
Bai QIN
1
;
Huai-Jin GUAN
2
Author Information
1. Eye Institute, Affiliated Hospital of Nantong University, Nantong 226000, China.
2. Eye Institute, Affiliated Hospital of Nantong University, Nantong 226000, China. Electronic address: guanhjeye@163.com.
- Publication Type:Journal Article
- Keywords:
Apoptosis;
Cataract;
Cell vitality;
Ganoderic acid A;
Lens epithelial cells
- MeSH:
Animals;
Apoptosis;
Cataract/prevention & control*;
Cell Line;
Cell Survival;
Epithelial Cells/radiation effects*;
Heptanoic Acids/pharmacology*;
Humans;
Lanosterol/pharmacology*;
Lens, Crystalline/radiation effects*;
Malondialdehyde/metabolism*;
Rats;
Superoxide Dismutase/metabolism*;
Ultraviolet Rays/adverse effects*
- From:
Chinese Journal of Natural Medicines (English Ed.)
2020;18(12):934-940
- CountryChina
- Language:English
-
Abstract:
A contributory role of oxidative stress and protection by antioxidant nutrients have been suspected in cataract formation. Ganoderic acid A (GAA), an effective lanostane triterpene, is widely reported as an antioxidant. The aim of this study is to investigate the potential effects of GAA on cataract formation. After lens epithelial cells (LECs) were exposed to UVB radiation for different periods, cell viability, apoptosis-related protein levels, malondialdehyde (MDA) and superoxide dismutase (SOD) activities were monitored. We found that cell viability, the Bcl-2/Bax ratio and SOD activity were increased, while Cleaved caspase-3 levels and MDA activity were decreased compared with those in UVB-impaired LECs after GAA treated. Furthermore, GAA activated PI3K/AKT in UVB-impaired LECs and effectively delayed the occurrence of lens opacity in vitro. In conclusion, these findings demonstrated that GAA exhibited protective functions in SRA01/04 cells and rat lenses against UVB-evoked impairment through elevating cell viability and antioxidant activity, inhibiting cell apoptosis, activating the PI3K/AKT pathway and delaying lens opacity.
