Effect of quercetin on chondrocyte phenotype and extracellular matrix expression.
10.1016/S1875-5364(20)60036-X
- Author:
Zhi-Peng GUI
1
,
2
;
Yue HU
3
,
4
;
Yu-Ning ZHOU
3
,
4
;
Kai-Li LIN
1
,
5
;
Yuan-Jin XU
3
,
6
Author Information
1. Department of Oral & Cranio-maxillofacial Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200000, China
2. National Clinical Research Center for Oral Diseases, Shanghai Key Laboratory of Stomatology & Shanghai Research Institute of Stomatology, Shanghai 200000, China.
3. National Clinical Research Center for Oral Diseases, Shanghai Key Laboratory of Stomatology & Shanghai Research Institute of Stomatology, Shanghai 200000, China
4. Department of Oral Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200000, China.
5. National Clinical Research Center for Oral Diseases, Shanghai Key Laboratory of Stomatology & Shanghai Research Institute of Stomatology, Shanghai 200000, China. Electronic address: lklecnu@aliyun.com.
6. Department of Oral Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200000, China. Electronic address: drxuyuanjin@126.com.
- Publication Type:Journal Article
- Keywords:
Cartilage Regeneration;
Chondrocyte Phenotype;
Quercetin;
Silk protein
- MeSH:
Animals;
Cartilage/cytology*;
Chondrocytes/drug effects*;
Chondrogenesis/drug effects*;
Extracellular Matrix/metabolism*;
Quercetin/pharmacology*;
Rats;
Signal Transduction/drug effects*;
Tissue Scaffolds
- From:
Chinese Journal of Natural Medicines (English Ed.)
2020;18(12):922-933
- CountryChina
- Language:English
-
Abstract:
Due to the poor repair ability of cartilage tissue, regenerative medicine still faces great challenges in the repair of large articular cartilage defects. Quercetin is widely applied as a traditional Chinese medicine in tissue regeneration including liver, bone and skin tissues. However, the evidence for its effects and internal mechanisms for cartilage regeneration are limited. In the present study, the effects of quercetin on chondrocyte function were systematically evaluated by CCK8 assay, PCR assay, cartilaginous matrix staining assays, immunofluorescence assay, and western blotting. The results showed that quercetin significantly up-regulated the expression of chondrogenesis genes and stimulated the secretion of GAG (glycosaminoglycan) through activating the ERK, P38 and AKT signalling pathways in a dose-dependent manner. Furthermore, in vivo experiments revealed that quercetin-loaded silk protein scaffolds dramatically stimulated the formation of new cartilage-like tissue with higher histological scores in rat femoral cartilage defects. These data suggest that quercetin can effectively stimulate chondrogenesis in vitro and in vivo, demonstrating the potential application of quercetin in the regeneration of cartilage defects.
