Rnf20 deficiency in adipocyte impairs adipose tissue development and thermogenesis.
10.1007/s13238-020-00770-2
- Author:
Xiaojuan LIANG
1
;
Cong TAO
1
;
Jianfei PAN
1
;
Lilan ZHANG
1
;
Lulu LIU
1
;
Ying ZHAO
1
;
Yiping FAN
1
;
Chunwei CAO
2
;
Jiali LIU
1
;
Jin ZHANG
3
;
Sin Man LAM
4
;
Guanghou SHUI
4
;
Wanzhu JIN
2
;
Wei LI
2
;
Jianguo ZHAO
5
;
Kui LI
6
;
Yanfang WANG
7
Author Information
1. State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.
2. Institute of Zoology, Chinese Academy of Sciences, Beijing, 100101, China.
3. College of Biological, Chemical Sciences and Engineering, Jiaxing University, Jiaxing, 314001, China.
4. Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100101, China.
5. Institute of Zoology, Chinese Academy of Sciences, Beijing, 100101, China. zhaojg@ioz.ac.cn.
6. State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, 100193, China. kuili@caas.cn.
7. State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, 100193, China. wangyanfang@caas.cn.
- Publication Type:Journal Article
- Keywords:
RNF20;
adipose tissue development;
fat loss;
thermogenesis
- From:
Protein & Cell
2021;12(6):475-492
- CountryChina
- Language:English
-
Abstract:
RNF20, an E3 ligase critical for monoubiquitination of histone H2B at lysine 120 (H2Bub), has been implicated in the regulation of various cellar processes; however, its physiological roles in adipocytes remain poorly characterized. Here, we report that the adipocyte-specific knockout of Rnf20 (ASKO) in mice led to progressive fat loss, organomegaly and hyperinsulinemia. Despite signs of hyperinsulinemia, normal insulin sensitivity and improved glucose tolerance were observed in the young and aged CD-fed ASKO mice. In addition, high-fat diet-fed ASKO mice developed severe liver steatosis. Moreover, we observed that the ASKO mice were extremely sensitive to a cold environment due to decreased expression levels of brown adipose tissue (BAT) selective genes, including uncoupling protein 1 (Ucp1), and impaired mitochondrial functions. Significantly decreased levels of peroxisome proliferator-activated receptor gamma (Pparγ) were observed in the gonadal white adipose tissues (gWAT) from the ASKO mice, suggesting that Rnf20 regulates adipogenesis, at least in part, through Pparγ. Rosiglitazone-treated ASKO mice exhibited increased fat mass compared to that of the non-treated ASKO mice. Collectively, our results illustrate the critical role of RNF20 in control of white and brown adipose tissue development and physiological function.
