TRIM35 mediates protection against influenza infection by activating TRAF3 and degrading viral PB2.
10.1007/s13238-020-00734-6
- Author:
Nan SUN
1
;
Li JIANG
1
;
Miaomiao YE
1
;
Yihan WANG
1
;
Guangwen WANG
1
;
Xiaopeng WAN
1
;
Yuhui ZHAO
1
;
Xia WEN
1
;
Libin LIANG
1
;
Shujie MA
1
;
Liling LIU
1
;
Zhigao BU
1
;
Hualan CHEN
2
;
Chengjun LI
3
Author Information
1. State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, 150069, China.
2. State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, 150069, China. chenhualan@caas.cn.
3. State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, 150069, China. lichengjun@caas.cn.
- Publication Type:Research Support, Non-U.S. Gov't
- Keywords:
PB2;
TRAF3;
TRIM35;
antiviral immunity;
influenza A virus;
ubiquitination
- MeSH:
A549 Cells;
Animals;
Apoptosis Regulatory Proteins/immunology*;
DEAD Box Protein 58/immunology*;
Dogs;
HEK293 Cells;
Humans;
Influenza A Virus, H1N1 Subtype/immunology*;
Madin Darby Canine Kidney Cells;
Mice;
Mice, Knockout;
Orthomyxoviridae Infections/pathology*;
Proteolysis;
RAW 264.7 Cells;
Signal Transduction/immunology*;
THP-1 Cells;
TNF Receptor-Associated Factor 3/immunology*;
Ubiquitination/immunology*;
Viral Proteins/immunology*
- From:
Protein & Cell
2020;11(12):894-914
- CountryChina
- Language:English
-
Abstract:
Tripartite motif (TRIM) family proteins are important effectors of innate immunity against viral infections. Here we identified TRIM35 as a regulator of TRAF3 activation. Deficiency in or inhibition of TRIM35 suppressed the production of type I interferon (IFN) in response to viral infection. Trim35-deficient mice were more susceptible to influenza A virus (IAV) infection than were wild-type mice. TRIM35 promoted the RIG-I-mediated signaling by catalyzing Lys63-linked polyubiquitination of TRAF3 and the subsequent formation of a signaling complex with VISA and TBK1. IAV PB2 polymerase countered the innate antiviral immune response by impeding the Lys63-linked polyubiquitination and activation of TRAF3. TRIM35 mediated Lys48-linked polyubiquitination and proteasomal degradation of IAV PB2, thereby antagonizing its suppression of TRAF3 activation. Our in vitro and in vivo findings thus reveal novel roles of TRIM35, through catalyzing Lys63- or Lys48-linked polyubiquitination, in RIG-I antiviral immunity and mechanism of defense against IAV infection.
