Mapping the epigenetic modifications of DNA and RNA.
10.1007/s13238-020-00733-7
- Author:
Lin-Yong ZHAO
1
;
Jinghui SONG
2
;
Yibin LIU
3
;
Chun-Xiao SONG
4
;
Chengqi YI
5
Author Information
1. Department of Gastrointestinal Surgery and Laboratory of Gastric Cancer, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, and Collaborative Innovation Center for Biotherapy, Chengdu, 610041, China.
2. State Key Laboratory of Protein and Plant Gene Research, School of Life Sciences, Peking University, Beijing, 100871, China.
3. Ludwig Institute for Cancer Research, Nuffield Department of Medicine, University of Oxford, Oxford, OX3 7FZ, UK.
4. Ludwig Institute for Cancer Research, Nuffield Department of Medicine, University of Oxford, Oxford, OX3 7FZ, UK. chunxiao.song@ludwig.ox.ac.uk.
5. State Key Laboratory of Protein and Plant Gene Research, School of Life Sciences, Peking University, Beijing, 100871, China. chengqi.yi@pku.edu.cn.
- Publication Type:Review
- Keywords:
DNA methylation;
DNA modification;
RNA modification;
epigenetics;
epitranscriptomics;
long read sequencing
- MeSH:
Animals;
DNA/metabolism*;
DNA Methylation;
Epigenesis, Genetic;
Epigenomics;
Humans;
RNA/metabolism*;
Transcriptome
- From:
Protein & Cell
2020;11(11):792-808
- CountryChina
- Language:English
-
Abstract:
Over 17 and 160 types of chemical modifications have been identified in DNA and RNA, respectively. The interest in understanding the various biological functions of DNA and RNA modifications has lead to the cutting-edged fields of epigenomics and epitranscriptomics. Developing chemical and biological tools to detect specific modifications in the genome or transcriptome has greatly facilitated their study. Here, we review the recent technological advances in this rapidly evolving field. We focus on high-throughput detection methods and biological findings for these modifications, and discuss questions to be addressed as well. We also summarize third-generation sequencing methods, which enable long-read and single-molecule sequencing of DNA and RNA modification.
