Three-dimensional reconstruction of systematic histological sections: application to observations on palatal shelf elevation.
10.1038/s41368-021-00122-8
- Author:
Weilong LIU
1
;
Xiaoming WANG
1
;
Yinuo WANG
2
;
Yahong WANG
1
;
Jing ZHANG
2
;
Bing SHI
3
;
Chenghao LI
4
Author Information
1. State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases & Department of Cleft Lip and Palate Surgery, West China School of Stomatology, Sichuan University, Chengdu, China.
2. College of Electrical Engineering and information Technology, Sichuan University, Chengdu, China.
3. State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases & Department of Cleft Lip and Palate Surgery, West China School of Stomatology, Sichuan University, Chengdu, China. shibingcn@vip.sina.com.
4. State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases & Department of Cleft Lip and Palate Surgery, West China School of Stomatology, Sichuan University, Chengdu, China. leechenghao_cn@yahoo.com.
- Publication Type:Journal Article
- From:
International Journal of Oral Science
2021;13(1):17-17
- CountryChina
- Language:English
-
Abstract:
Normal mammalian secondary palate development undergoes a series of processes, including palatal shelf (PS) growth, elevation, adhesion and fusion, and palatal bone formation. It has been estimated that more than 90% of isolated cleft palate is caused by defects associated with the elevation process. However, because of the rapidly completed elevation process, the entire process of elevation will never be easy to clarify. In this article, we present a novel method for three-dimensional (3D) reconstruction of thick tissue blocks from two-dimensional (2D) histological sections. We established multiplanar sections of the palate and tongue in coronal and sagittal directions, and further performed 3D reconstruction to observe the morphological interaction and connection between the two components prior to and during elevation. The method completes an imaging system for simultaneous morphological analysis of thick tissue samples using both synthetic and real data. The new method will provide a comprehensive picture of reorientation morphology and gene expression pattern during the palatal elevation process.
