USP34 regulates tooth root morphogenesis by stabilizing NFIC.
10.1038/s41368-021-00114-8
- Author:
Shuang JIANG
1
;
Rui SHENG
1
;
Xingying QI
1
;
Jun WANG
1
;
Yuchen GUO
2
;
Quan YUAN
3
Author Information
1. State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases & West China Hospital of Stomatology, Sichuan University, Chengdu, China.
2. State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases & West China Hospital of Stomatology, Sichuan University, Chengdu, China. guoyuchen@scu.edu.cn.
3. State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases & West China Hospital of Stomatology, Sichuan University, Chengdu, China. yuanquan@scu.edu.cn.
- Publication Type:Research Support, Non-U.S. Gov't
- MeSH:
Cell Differentiation;
Female;
Morphogenesis;
NFI Transcription Factors;
Odontogenesis;
Tooth Root
- From:
International Journal of Oral Science
2021;13(1):7-7
- CountryChina
- Language:English
-
Abstract:
Tooth root morphogenesis involves two biological processes, root elongation and dentinogenesis, which are guaranteed by downgrowth of Hertwig's epithelial root sheath (HERS) and normal odontoblast differentiation. Ubiquitin-dependent protein degradation has been reported to precisely regulate various physiological processes, while its role in tooth development is still elusive. Here we show ubiquitin-specific protease 34 (USP34) plays a pivotal role in root formation. Deletion of Usp34 in dental mesenchymal cells leads to short root anomaly, characterized by truncated roots and thin root dentin. The USP34-deficient dental pulp cells (DPCs) exhibit decreased odontogenic differentiation with downregulation of nuclear factor I/C (NFIC). Overexpression of NFIC partially restores the impaired odontogenic potential of DPCs. These findings indicate that USP34-dependent deubiquitination is critical for root morphogenesis by stabilizing NFIC.
