Ginsenoside 20(S)-Rg3 upregulates tumor suppressor VHL gene expression by suppressing DNMT3A-mediated promoter methylation in ovarian cancer cells.
10.12122/j.issn.1673-4254.2021.01.14
- Author:
Lijie WANG
1
;
Xi HAN
2
;
Xia ZHENG
3
;
Yuanyuan ZHOU
4
;
Huilian HOU
5
;
Wei CHEN
6
;
Xu LI
1
;
Le ZHAO
1
Author Information
1. Center for Translational Medicine, the First Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710061, China.
2. Department of Obstetrics and Gynecology, Shaanxi Provincial People's Hospital, Xi'an 710068, China.
3. the Second Affiliated Hospital of Zhejiang University School of medicine, Hangzhou 310009, China.
4. Department of Obstetrics and Gynecology, the First Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710061, China.
5. Department of Pathology, the First Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710061, China.
6. Center for Laboratory Medicine, the First Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710061, China.
- Publication Type:Journal Article
- Keywords:
ginsenoside;
methylation;
ovarian cancer;
von Hippel-Lindau
- MeSH:
Animals;
Cell Line, Tumor;
DNA Methylation;
Female;
Gene Expression;
Ginsenosides/pharmacology*;
Humans;
Mice;
Mice, Nude;
Ovarian Neoplasms/genetics*;
Promoter Regions, Genetic;
Von Hippel-Lindau Tumor Suppressor Protein/genetics*
- From:
Journal of Southern Medical University
2021;41(1):100-106
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To explore the mechanism by which ginsenoside 20(S)-Rg3 upregulates the expression of tumor suppressor von Hippel-Lindau (VHL) gene in ovarian cancer cells.
METHODS:Ovarian cancer cell line SKOV3 treated with 20(S)-Rg3 were examined for mRNA and protein levels of VHL, DNMT1, DNMT3A and DNMT3B by real-time PCR and Western blotting, respectively. The changes in VHL mRNA expression in SKOV3 cells in response to treatment with 5-Aza-CdR, a DNA methyltransferase inhibitor, were detected using real-time PCR. VHL gene promoter methylation was examined with methylation-specific PCR and VHL expression levels were determined with real-time PCR and Western blotting in non-treated or 20(S)-Rg3-treated SKOV3 cells and in 20(S)-Rg3-treated DNMT3A-overexpressing SKOV3 cells. VHL and DNMT3A protein levels were detected by immunohistochemistry in subcutaneous SKOV3 cell xenografts in nude mice.
RESULTS:Treatment of SKOV3 cells with 20(S)-Rg3 significantly upregulated VHL and downregulated DNMT3A expressions at both the mRNA and protein levels (
CONCLUSIONS:Ginsenoside 20(S)-Rg3 upregulates VHL expression in ovarian cancer cells by suppressing DNMT3A-mediated DNA methylation.
