Characteristics of cervical microecology in late reproductive-age women with different grades of cervical lesions.
10.12122/j.issn.1673-4254.2020.12.11
- Author:
Qingzhi ZHAI
1
;
Tengjie REN
1
;
Yurong FU
1
;
Zhe ZHANG
1
;
Li'an LI
1
;
Yali LI
1
;
Yuanguang MENG
1
Author Information
1. Department of Obstetrics and Gynecology, First Medical Center, General Hospital of PLA, Beijing 100853, China.
- Publication Type:Journal Article
- Keywords:
16S rRNA sequencing analysis;
cervical lesions;
cervical microecology;
microbiome
- MeSH:
Female;
Humans;
Microbiota;
Papillomaviridae;
Papillomavirus Infections;
RNA, Ribosomal, 16S/genetics*;
Uterine Cervical Neoplasms;
Vaginal Smears
- From:
Journal of Southern Medical University
2020;40(12):1768-1775
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To analyze the characteristics of cervical microecology in late reproductive-age women with cervical lesions and explore new methods for preventing cervical lesions.
METHODS:Cervical smears were obtained from a total of 147 women of late reproductive age, including 24 with high-risk HPV infection (HR-HPV), 27 with low-grade squamous intra-epithelial lesions (LSIL), 36 with high-grade squamous intra-epithelial lesions (HSIL), 35 with cervical cancer (CC) and 25 healthy women. llumina MiSeq sequencing of V3-V4 region of the 16S rRNA gene amplicons was used to characterize the vaginal microbiota of the women. OTUs analysis of the valid data was performed, and the α-diversity (Chao1, Simpson's Index and Shannon Index) and β-diversity (T-test, weighted UniFrac β diversity, and MetaStat analysis) were evaluated.
RESULTS:Dilution curve and species accumulation boxplot validated the quality of the samples. OTUs analysis of the 5 groups demonstrated that cervical bacterial genus consisted primarily of
CONCLUSIONS:The abundance of
