Effect of exosomes derived from human Epstein-Barr virus-positive nasopharyngeal carcinoma cells on lymphangiogenesis and lymph node metastasis.
10.12122/j.issn.1673-4254.2020.12.12
- Author:
Xingrui CHEN
1
;
Dengke LI
2
;
Zhongxi HUANG
2
;
Shuisheng ZHONG
1
;
Linbo CAI
1
Author Information
1. Guangdong Sanjiu Brain Hospital, Guangzhou 510510, China.
2. Cancer Research Institute, Southern Medical University, Guangzhou 510515, China.
- Publication Type:Journal Article
- Keywords:
Epstein-Barr virus;
exosome;
lymph node metastasis;
lymphangiogenesis;
nasopharyngeal carcinoma;
tumor microenvironment
- MeSH:
Animals;
Cell Line, Tumor;
Endothelial Cells;
Epstein-Barr Virus Infections;
Exosomes;
Herpesvirus 4, Human;
Humans;
Lymphangiogenesis;
Lymphatic Metastasis;
Mice;
Mice, Nude;
Nasopharyngeal Carcinoma;
Nasopharyngeal Neoplasms
- From:
Journal of Southern Medical University
2020;40(12):1776-1783
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the effect of exosomes derived from Epstein-Barr virus (EBV)-positive nasopharyngeal carcinoma (NPC) cells on lymphangiogenesis and lymph node metastasis of NPC.
METHODS:Exosomes from NP69 cells and EBV-positive HK1 (HK1-EBV) cells were obtained by ultracentrifugation and identified by Western blotting and nanoparticle tracking analysis. Dio dye phagocytosis test was performed to observe exosome uptake by lymphatic endothelial cells. Lymphatic endothelial cells were treated with exosomes from nasopharyngeal epithelium (NP69), HK1-EBV, and C666-1 cells or exosome-free supernatant of HK1-EBV and C666-1 cells, and tube formation and migration of the cells were observed. In a nude mouse model of popliteal lymph node metastasis of NPC, the effects of normal saline, NP69 cell-derived exosomes, HK1-EBV cell-derived exosomes, exosome-free supernatant of HK1-EBV cells, and HK1-EBV exosome-free supernatant protein on lymphangiogenesis and lymph node metastasis of the tumor were observed.
RESULTS:The exosomes obtained by ultracentrifugation contained abundant exosome-specific proteins and showed a normal size range. The exosomes from NPC cells and NP69 cells could be taken up by lymphatic endothelial cells. Compared with the blank control and exosomes form NP69 cells, exosomes derived from HK1-EBV and C666-1 cells significantly promoted tube formation and migration of lymphatic endothelial cells (
CONCLUSIONS:Exosomes from EBV-positive NPC cells can significantly promote lymphangiogenesis and lymph node metastasis of NPC.
