MiR-4443 promotes migration and invasion of breast cancer cells by inhibiting PEBP1 expression.
10.12122/j.issn.1673-4254.2020.12.03
- Author:
Jinyan WANG
1
;
Jinqiu WANG
2
;
Quan GU
3
;
Yan YANG
1
;
Yajun MA
1
;
Jing ZHU
1
;
Quanan ZHANG
1
Author Information
1. Department of Oncology, The Affiliated Jiangning Hospital of Nanjing Medical University, Nanjing 211100, China.
2. Department of Oncology, Dafeng People's Hospital, Yancheng 224199, China.
3. Nanjing Medical University, Nanjing 211166, China.
- Publication Type:Journal Article
- Keywords:
breast neoplasms;
microRNAs;
neoplasm metastasis;
phosphatidylethanolamine binding protein 1
- MeSH:
Breast Neoplasms/genetics*;
Cell Line, Tumor;
Cell Movement;
Cell Proliferation;
Gene Expression Regulation, Neoplastic;
Humans;
MCF-7 Cells;
MicroRNAs/genetics*;
Neoplasm Invasiveness/genetics*;
Phosphatidylethanolamine Binding Protein
- From:
Journal of Southern Medical University
2020;40(12):1712-1719
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the effect of miR-4443 expression on migration and invasion of breast cancer.
METHODS:We examined the expression of miR-4443 in breast carcinoma in situ and paired adjacent tissues from 3 breast cancer patients with high-throughput sequencing and verified the results using TCGA database. We also detected miR-4443 expressions using real-time quantitative PCR (RT-qPCR) in low invasive and highly invasive breast cancer cells (MCF-7 and MDA-MB-231 cells, respectively). The changes in apoptosis, migration and invasion of MCF-7 and MDA-MB-231 cells after transfection with miR-4443 mimics, mimics-NC, miR-4443 inhibitor or inhibitor-NC were analyzed using flow cytometry, wound healing assay and Transwell invasion assay. The target gene of miR-4443 was predicted by bioinformatics software and validated by a dual luciferase reporter gene system. RT-qPCR and Western blotting were performed to detect the expression of recombinant human phosphatidyl ethanolamine binding protein 1 (PEBP1) in the transfected cells.
RESULTS:The expression of miR-4443 was significantly higher in the breast cancer tissues than in the adjacent tissues (
CONCLUSIONS:MiR-4443 promotes the migration and invasion of breast cancer cells by inhibiting the expression of PEBP1, suggesting the possibility of suppressing miR-4443 expression as a potential therapeutic strategy for breast cancer.
