ALKBH5 suppresses migration and invasion of human trophoblast cells by inhibiting epithelial-mesenchymal transition.
10.12122/j.issn.1673-4254.2020.12.04
- Author:
Jianping HE
1
;
Xiaojuan LI
2
;
Mengxin LÜ
1
;
Jue WANG
3
;
Jian TANG
1
;
Shengjun LUO
1
;
Yuan QIAN
1
Author Information
1. Department of Medical Genetics and Prenatal Diagnosis, Kunming Maternal and Child Healthcare Hospital, Kunming 650031, China.
2. Department of Obstetrics, First Affiliated Hospital of Kunming Medical University, Kunming 650032, China.
3. Prenatal Diagnosis Room, Clinical Laboratory, First Affiliated Hospital of Kunming Medical University, Kunming 650032, China.
- Publication Type:Journal Article
- Keywords:
ALKBH5;
epithelial-mesenchymal transition;
invasion;
migration;
preeclampsia
- MeSH:
AlkB Homolog 5, RNA Demethylase;
Cell Line, Tumor;
Cell Movement;
Epithelial-Mesenchymal Transition;
Female;
Humans;
Pre-Eclampsia;
Pregnancy;
Trophoblasts;
Vimentin/genetics*
- From:
Journal of Southern Medical University
2020;40(12):1720-1725
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the effects of ALKBH5 on migration, invasion and epithelial-mesenchymal transition (EMT) of human trophoblast cells.
METHODS:The expression plasmid of ALKBH5 or a negative control plasmid (ALKBH5-NC) was transfected in human trophoblast HTR-8 /SVneo cells, and the expressions of ALKBH5 mRNA and protein were detected by qRT-PCR and Western blotting. Transwell assay was used to assess the changes in migration and invasion abilities of the trophoblast cells after the transfection. Western blotting was performed to detect the expressions of EMT-related proteins in the cells including vimentin, fibronectin, E-cadherin, N-cadherin, MMP9 and MMP2.
RESULTS:ALKBH5 mRNA and protein expressions were significantly higher in ALKBH5 group than in the control group (
CONCLUSIONS:ALKBH5 is involved in the pathogenesis of preeclampsia by inhibiting EMT of trophoblast cells and hence reducing their migration and invasion abilities.
