Silencing long non-coding RNA HIF1A-AS2 inhibits proliferation, invasion and migration of cervical cancer cells
10.12122/j.issn.1673-4254.2020.11.12
- Author:
Rong DENG
1
;
Fuyun ZHANG
2
;
Fuzhen LEI
1
;
Wenjun LIU
1
;
Sisun LIU
3
;
Wenhua WANG
2
Author Information
1. Department of Gynecology, General Hospital of Pingxiang Mining Group CO., Ltd., Pingxiang 337003, China, China.
2. Department of Gynecology, Pingxiang People's Hospital, Pingxiang 337000, China.
3. Department of Obstetrics and Gynecology, First Affiliated Hospital of Nanchang University, Nanchang 330006, China.
- Publication Type:Journal Article
- Keywords:
cervical cancer;
epithelial-mesenchymal transition;
long non-coding RNA HIF1A-AS2;
tumor stem cell
- MeSH:
Cell Line, Tumor;
Cell Movement/genetics*;
Cell Proliferation/genetics*;
Epithelial-Mesenchymal Transition/genetics*;
Female;
Gene Expression Regulation, Neoplastic;
Humans;
Hypoxia-Inducible Factor 1, alpha Subunit;
RNA, Long Noncoding/genetics*;
RNA, Small Interfering/genetics*;
Uterine Cervical Neoplasms/genetics*
- From:
Journal of Southern Medical University
2020;40(11):1615-1621
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To explore the inhibitory effects of silencing long non-coding RNA (LncRNA) HIF1A-AS2 on epithelialmesenchymal transition (EMT) and tumor stem cell-like phenotype in cervical cancer cells.
METHODS:We designed 3 shRNA constructs for silencing HIF1A-AS2 in CaSki cells, and the shRNA with the strongest interference effect was selected for subsequent experiment. CaSki cells were transfected with shRNA-NC or Sh-HIF1A-AS2, and the changes in cell viability, invasion ability, EMT, expressions of EMT-related proteins, formation of cell spheres and expressions of stem cell markers were detected.
RESULTS:Transfection with shRNA-NC and Sh-HIF1A-AS2 did not significantly affected the viability of CaSki cells (
CONCLUSIONS:Silencing HIF1A-AS2 can inhibit proliferation, invasion and migration of cervical cancer cells
