Rosmarinic acid inhibits high glucose-induced cardiomyocyte hypertrophy by activating Parkin-mediated mitophagy.
10.12122/j.issn.1673-4254.2020.11.14
- Author:
Jiayu DIAO
1
;
Hongmou ZHAO
2
;
Yujie NING
3
;
Wenqi HAN
1
;
Yi WANG
1
;
Gong CHENG
1
;
Xiling SHOU
1
;
Hongjun YOU
1
Author Information
1. Department of Cardiovascular Medicine, Shaanxi Provincial People's Hospital, Xi'an 710068, China, China.
2. Department of Foot and Ankle Surgery, Xi'an Honghui Hospital, Xi'an 710016, China.
3. School of Public Health, Xi'an Jiaotong University Health Science Center, Xi'an 710061, China.
- Publication Type:Journal Article
- Keywords:
Parkin;
cardiomyocyte hypertrophy;
mitophagy;
oxidative stress;
rosmarinic acid
- MeSH:
Animals;
Cinnamates;
Depsides;
Glucose;
Hypertrophy;
Mitophagy;
Myocytes, Cardiac;
Protein Kinases;
Rats;
Reactive Oxygen Species;
Ubiquitin-Protein Ligases/genetics*
- From:
Journal of Southern Medical University
2020;40(11):1628-1633
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To evaluate the effect of rosmarinic acid (RA) on mitophagy and hypertrophy of cardiomyocytes exposed to high glucose (HG).
METHODS:Rat cardiomyocytes (H9c2) exposed to HG (25 mmol/L) were treated with 50 μmol/L RA or with both RA treatment and Parkin siRNA transfection, with the cells cultured in normal glucose (5.5 mmol/L) and HG as the controls. The expressions of PINK1, Parkin and LC3II/LC3I in the cells were detected by Western blotting. The formation of mitochondrial autophagosomes was observed by transmission electron microscope. Flow cytometry was employed to detect the level of reactive oxygen species (ROS) and apoptotic rate of the cells. The activities of respiratory chain complex enzymes were measured by spectrophotometry. Fluorescence enzyme labeling and
RESULTS:RA treatment significantly increased the expression levels of PINK1, Parkin and LC3-II/I (
CONCLUSIONS:RA can protect rat cardiomyocytes against oxidative stress injury and cardiomyocyte hypertrophy induced by HG by activating Parkin-mediated mitophagy.
