miR-324-5p inhibits lipopolysaccharide-induced proliferation of rat glomerular mesangial cells by regulating the Syk/Ras/c-fos pathway.
10.12122/j.issn.1673-4254.2020.11.06
- Author:
Jing WANG
1
;
Xiaoli ZHU
1
;
Xiujuan QIN
1
;
Hui JIANG
1
;
Yachen GAO
1
;
Jiarong GAO
1
Author Information
1. First Affiliated Hospital, Anhui University of Chinese Medicine, Hefei 230012, China.
- Publication Type:Journal Article
- Keywords:
Chronic glomerulonephritis;
Syk/Ras/c-fos signaling pathway;
cell proliferation;
miR-324-5p
- MeSH:
Animals;
Cell Proliferation;
Lipopolysaccharides;
Mesangial Cells;
MicroRNAs/genetics*;
Proto-Oncogene Proteins c-fos;
Rats;
Receptor Protein-Tyrosine Kinases;
Signal Transduction;
ras Proteins
- From:
Journal of Southern Medical University
2020;40(11):1571-1578
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the effect of miR-324-5p on the proliferation of rat glomerular mesangial (HBZY-1) cells and the role of Syk/Ras/c-fos signaling pathway in mediating this effect.
METHODS:HBZY-1 cells cultured in vitro were transiently transfected with miR-324-5p mimics or miR-324-5p-mimics-NC followed by treatment with lipopolysaccharide (LPS). MTT assay was used to detect the proliferation activity of HBZY-1 cells, and RT-qPCR was used to detect the expressions of miR-324-5p and the mRNA expressions of Syk, Ras, MEK1/2, ERK1/2 and c-fos mRNA. The protein expressions of p-Syk, Ras, p-MEK1/2, p-ERK1/2 and c-Fos were detected by Western blotting and immunofluorescence assay.
RESULTS:MTT assay showed that exposure to LPS significantly enhanced the proliferative activity of HBZY-1 cells. Compared with the cells treated with LPS and LPS + mimics NC, the cells transfected with miR-324-5p mimics prior to LPS exposure exhibited significantly lowered proliferative activity. Transfection with miR-324-5p mimics significantly lowered the mRNA expressions of Syk, Ras, MEK1/2, ERK1/2 and c-fos and the protein expressions of p-Syk, Ras, MEK1/2, ERK1/2 and c-Fos (
CONCLUSIONS:miR-324-5p can inhibit the proliferation of rat chronic glomerulonephritis cells induced by LPS by inhibiting Syk/Ras/c-fos signaling pathway and may potentially serve as a diagnostic indicator and a therapeutic target for chronic glomerulonephritis.
