Expression of pituitary tumor-transforming gene-1 and its pathogenic role in systemic sclerosis.
10.12122/j.issn.1673-4254.2020.11.05
- Author:
Anqiao YANG
1
;
Yan HUANG
1
;
Yuting ZHANG
1
;
Kai YANG
2
;
Jiucun WANG
1
;
Qingmei LIU
3
Author Information
1. School of Life Sciences, Fudan University, Shanghai 200433, China.
2. Department of Dermatology, Jing'an District Central Hospital, Shanghai 200040, China.
3. Department of Dermatology, Huashan Hospital Affiliated to Fudan University, Shanghai 200040, China.
- Publication Type:Journal Article
- Keywords:
cell proliferation;
fibroblasts;
fibrosis;
systemic sclerosis;
tumor-transforming gene-1
- MeSH:
Cells, Cultured;
Fibroblasts;
Fibrosis;
Humans;
Scleroderma, Systemic/pathology*;
Securin;
Skin/pathology*
- From:
Journal of Southern Medical University
2020;40(11):1564-1570
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the expression of tumor-transforming gene-1 (PTTG1) in systemic sclerosis (SSc) and its role in fibrosis.
METHODS:Skin biopsy samples were collected from 21 patients with SSc and 22 patients with healthy skin for detecting the mRNA and protein expressions of PTTG1 using real-time PCR (RT-PCR) and immunohistochemistry, respectively. In cultured primary human dermal fibroblasts, PTTG1 expression was knocked down via RNA interference (siRNA), and the mRNA expression levels of PTTG1 and the fibrosis-related genes
RESULTS:Compared with those in normal skin samples, the mRNA and protein expressions of PTTG1 increased significantly in the skin tissue of patients with SSc (
CONCLUSIONS:PTTG1 is highly expressed in skin tissues of patients with SSc, and PTTG1 knockdown can reduce the activity of the dermal fibroblasts, suggesting a close correlation of PTTG1 with fibrosis in SSc.
