Expression of CDC25A in non-small cell lung cancer and its relationship with let-7 gene.
10.12122/j.issn.1673-4254.2020.11.13
- Author:
Dianming LI
1
;
Zhaofei LIU
2
;
Guolan NING
3
Author Information
1. Department of Respiratory and Critical Medicine, First Affiliated Hospital of Bengbu Medical College, Bengbu 233000, China.
2. Department of Respiratory Medicine, Linquan County People's Hospital, Linquan 236400, China.
3. Department of Respiratory and Critical Care Medicine, Fuyang Second People's Hospital, Fuyang 236000, China.
- Publication Type:Journal Article
- Keywords:
CDC25A;
let-7;
microRNA;
non-small cell lung cancer
- MeSH:
Carcinoma, Non-Small-Cell Lung/genetics*;
Gene Expression Regulation, Neoplastic;
Humans;
Lung;
Lung Neoplasms/genetics*;
Lymphatic Metastasis;
MicroRNAs;
RNA, Messenger/genetics*;
cdc25 Phosphatases
- From:
Journal of Southern Medical University
2020;40(11):1622-1627
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the expression of CDC25A in non- small cell lung cancer (NSCLC) tissues and explore its correlation with the clinicpathological features of the patients and the expressions of let-7a1 and let-7c.
METHODS:We collected surgical specimens of pathologically confirmed NSCLC tissues and paired adjacent lung tissues from 44 patients and tissues of benign lung lesions from 9 patients. The expressions of CDC25A protein and mRNA in the tissues were detected by immunohistochemistry and fluorescence quantitative RT-PCR, respectively; the expressions of let-7a1 and let-7c mRNA were detected using tail-adding fluorescence quantitative RT-PCR.
RESULTS:The positivity rate of CDC25A protein expression was significantly higher in NSCLC tissues than in the adjacent tissues and benign pulmonary lesions (
CONCLUSIONS:The expression level of CDC25A is significantly increased in NSCLC with a negative correlation with Let-7c expression, which identifies CDC25A as a possible downstream target gene of Let-7c.
