Association of JMJD3, MMP-2 and VEGF expressions with clinicopathological features of invasive ductal breast carcinoma.
10.12122/j.issn.1673-4254.2020.11.09
- Author:
Xiaoyan XU
1
;
Jianjun WANG
1
;
Chen YAN
1
;
Yingli MEN
2
;
Huang JIANG
1
;
Huijuan FANG
1
;
Xianwei XU
1
;
Jinhua YANG
1
Author Information
1. Department of Pathology, People's Hospital of Zhengzhou, Zhengzhou 450000, China.
2. Academician Workstation, People's Hospital of Zhengzhou, Zhengzhou 450000, China.
- Publication Type:Journal Article
- Keywords:
JMJD3;
clinicopathological features;
invasive ductal carcinoma of breast;
matrix metalloproteinase-2;
vascular endothelial growth factor
- MeSH:
Breast Neoplasms/genetics*;
Carcinoma, Ductal, Breast/genetics*;
Humans;
Jumonji Domain-Containing Histone Demethylases;
Lymphatic Metastasis;
Matrix Metalloproteinase 2;
Prognosis;
Vascular Endothelial Growth Factor A
- From:
Journal of Southern Medical University
2020;40(11):1593-1600
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To examine the expressions of JMJD3, matrix metalloproteinase-2 (MMP-2) and vascular endothelial growth factor (VEGF) in invasive ductal breast carcinoma, their association with the clinicopathological features of the patients and the effect of JMJD3 overexpression on proliferation and MMP-2 and VEGF expressions in breast cancer cells.
METHODS:The protein and mRNA expressions of JMJD3, MMP-2, and VEGF in invasive ductal breast carcinoma and paired adjacent tissues were detected by immunohistochemistry and RT-PCR, respectively, and their correlation with the clinicopathological characteristics of the patients was analyzed. Kaplan-Meier survival analysis was used to evaluate the correlation of JMJD3, MMP-2 and VEGF expression levels with the survival of the patients. In breast cancer MDA-MB-231 cells transfected with a JMJD3-expression plasmid, the expression of Ki67 was examined immunohistochemically, the cell proliferation was assessed with CCK8 assay, and the mRNA expressions of MMP-2 and VEGF were detected with RT-PCR.
RESULTS:Breast cancer tissues had significantly lower JMJD3 expression and higher MMP-2 and VEGF expressions at both the mRNA and protein levels than the adjacent tissue (
CONCLUSIONS:The expressions of JMJD3, MMP-2 and VEGF in invasive ductal breast carcinoma are closely correlated to tumor proliferation, invasion, metastasis and prognosis and can be used for prognostic evaluation of breast cancer.
