Effect of small interfering RNA-mediated BIRC6 silencing on apoptosis and autophagy of renal cancer 786-O cells.
10.12122/j.issn.1673-4254.2020.11.18
- Author:
Kaihua ZHONG
1
;
Dong CHEN
2
;
Zhiming WU
2
;
Xiaohong WANG
3
;
Bin PAN
4
;
Nanhui CHEN
1
;
Weifeng ZHONG
1
Author Information
1. Department of Urology, Meizhou People's Hospital, Meizhou 514031, China.
2. Department of Urology, Sun Yat-sen Cancer Center, Guangzhou 510060, China.
3. Department of Nephrology, Third Affiliated Hospital of Southern Medical University, Guangzhou 510630, China.
4. Department of Urology, First Affiliated Hospital of Jinan University, Guangzhou 510630, China.
- Publication Type:Journal Article
- Keywords:
BIRC6;
autophagy;
drug resistance;
renal cancer
- MeSH:
Apoptosis;
Autophagy;
Cell Line, Tumor;
Cell Proliferation;
Humans;
Inhibitor of Apoptosis Proteins/genetics*;
Kidney Neoplasms/genetics*;
RNA, Small Interfering/genetics*
- From:
Journal of Southern Medical University
2020;40(11):1651-1655
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To study the expression of BIRC6 in renal cancer tissues and investigate the effect of BIRC6 silencing on apoptosis and autophagy of 786-O cells.
METHODS:Twenty surgical specimens of renal cancer tissues and adjacent renal tissues were collected from Meizhou People's Hospital between February, 2016 and December, 2018 for detection of BIRC6 protein expression using immunohistochemistry. Renal cancer 786-O cells were transfected with a control small interfering RNA (siRNA) or BIRC6 siRNA
RESULTS:The expression of BIRC6 protein was significantly higher in renal cancer tissues than in the adjacent renal tissues. Western blotting showed that siRNA-mediated silencing of BIRC6 significantly lowered the expression of BIRC6 in 786-O cells. In the cells with BIRC6 silencing, treatment with 12.5, 25, 50, 100 and 200 μg/mL 5-FU resulted in significantly higher proliferation inhibition rates than in the cells transfected with the control siRNA (
CONCLUSIONS:Interference of BIRC6 mediated by siRNA can inhibit autophagy and promote 5-FU-induced apoptosis to enhance the sensitivity of 786-O cells to 5-FU.
