Effect of high-fat diet and exercise on asprosin and CTRP6 expression in subcutaneous and retroperitoneal adipose tissues in rats during mid-gestation.
10.12122/j.issn.1673-4254.2020.10.05
- Author:
Zhao YANG
1
;
Jianan JIANG
1
;
Jiaqi HUANG
1
;
Yujia ZHAO
1
;
Xiao LUO
1
;
Lin SONG
1
Author Information
1. Department of Physiology and Pathophysiology, School of Basic Medicine, Key Laboratory of Environment and Genes Related to Diseases of Ministry of Education, Xi'an Jiaotong University, Xi'an 710061, China.
- Publication Type:Journal Article
- Keywords:
CTRP6;
adipose tissue;
asprosin;
exercise during pregnancy;
high-fat diet
- MeSH:
Adipokines;
Animals;
Blood Glucose;
Diet, High-Fat/adverse effects*;
Female;
Intra-Abdominal Fat;
Pregnancy;
Rats;
Rats, Sprague-Dawley
- From:
Journal of Southern Medical University
2020;40(10):1406-1414
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To study the effects of high-fat (HF) diet and exercise on the expressions of asprosin and CTRP6 in adipose tissues in different regions of rats during mid-gestation.
METHODS:Pregnant SD rats were fed on a standard chow diet or a high-fat (60% fat content) diet for 14 days starting on gestation day (GD) 1. Starting from GD3, the rats fed either on normal or high-fat diet in the exercise groups (CH-RW and HF-RW groups) were allowed access to the running wheels for voluntary running, and those in sedentary groups (CH-SD and HF-SD groups) remained sedentary. At the end of the 14 days, adipose tissues were sampled from different regions of the rats for detecting the mRNA and protein expressions of asprosin and CTRP6 using RT-qPCR and Western blotting.
RESULTS:The mRNA expression of asprosin in retroperitoneal adipose tissues was significantly higher in HF-RW group than in the other 3 groups (
CONCLUSIONS:High-fat diet and exercise during mid-gedtation can affect the expression levels of asprosin and CTRP6 in adipose tissues of rats in a site-specific manner.