Menthol enhances interleukin-13-induced synthesis and secretion of mucin 5AC in human bronchial epithelial cells.
10.12122/j.issn.1673-4254.2020.10.08
- Author:
Mingyang ZHANG
1
;
Jing WANG
1
;
Minchao LI
1
Author Information
1. Department of Respiratory Medicine, Second Affiliated Hospital, Chongqing Medical University, Chongqing 400010, China.
- Publication Type:Journal Article
- Keywords:
anti-apoptotic protein;
bronchialasthma;
interleukin-13;
menthol;
mucin 5AC;
transient receptor potential melastatin 8
- MeSH:
Bronchi;
Epithelial Cells/drug effects*;
Humans;
Interleukin-13;
Menthol/pharmacology*;
Mucin 5AC
- From:
Journal of Southern Medical University
2020;40(10):1432-1438
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the effect of interleukin (IL) -13 combined with cold stimulation on synthesis and secretion of mucin (MUC) 5AC in human bronchial epithelial cell line 16HBE and explore the role of transient receptor potential 8 (TRPM8) and anti-apoptotic factor B-cell lymphoblast-2 (Bcl-2) in this process.
METHODS:16HBE cells were stimulated with 10 ng/mL IL-13, 1 mmol/L menthol, or both (1 mmol/L menthol was added after 6 days of IL-13 stimulation), and the changes in the expression of MUC5AC, intracellular Ca
RESULTS:The mRNA and protein expressions of MUC5AC increased significantly in 16HBE cells following stimulation with IL-13, menthol, and both (
CONCLUSIONS:Menthol combined with IL-13 produces a synergistic effect to promote the synthesis and secretion of MUC5AC in 16HBE cells possibly by activating TRPM8 receptor to upregulate the expression of Bcl-2.
