Osteogenic effects of antihypertensive drug benidipine on mouse MC3T3-E1 cells in vitro.

Baixiang Wang; Jiakang Yang; Lijie Fan; Yu Wang; Chenqiu Zhang; Huiming Wang

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Osteogenic effects of antihypertensive drug benidipine on mouse MC3T3-E1 cells in vitro.

Author: Baixiang WANG ¹ ; Jiakang YANG ² ; Lijie FAN ¹ ; Yu WANG ¹ ; Chenqiu ZHANG ¹ ; Huiming WANG ³
Author Information

1. The Affiliated Hospital of Stomatology, School of Stomatology, Zhejiang University School of Medicine, and Key Laboratory of Oral Biomedical Research of Zhejiang Province, Hangzhou 310006, China.
2. School of Stomatology, Zhejiang University School of Medicine, Hangzhou 310006, China.
3. The Affiliated Hospital of Stomatology, School of Stomatology, Zhejiang University School of Medicine, and Key Laboratory of Oral Biomedical Research of Zhejiang Province, Hangzhou 310006, China. whmwhm@zju.edu.cn.
Publication Type:Journal Article
Keywords: Benidipine; MC3T3-E1; Osteoblast; Osteogenesis
From: Journal of Zhejiang University. Science. B 2021;22(5):410-420
CountryChina
Language:English
Abstract: Hypertension is a prevalent systemic disease in the elderly, who can suffer from several pathological skeletal conditions simultaneously, including osteoporosis. Benidipine (BD), which is widely used to treat hypertension, has been proved to have a beneficial effect on bone metabolism. In order to confirm the osteogenic effects of BD, we investigated its osteogenic function using mouse MC3T3-E1 preosteoblast cells in vitro. The proliferative ability of MC3T3-E1 cells was significantly associated with the concentration of BD, as measured by methylthiazolyldiphenyl-tetrazolium bromide (MTT) assay and cell cycle assay. With BD treatment, the osteogenic differentiation and maturation of MC3T3-E1 cells were increased, as established by the alkaline phosphatase (ALP) activity test, matrix mineralized nodules formation, osteogenic genetic test, and protein expression analyses. Moreover, our data showed that the BMP2/Smad pathway could be the partial mechanism for the promotion of osteogenesis by BD, while BD might suppress the possible function of osteoclasts through the OPG/RANKL/RANK (receptor activator of nuclear factor-κB (NF-κB)) pathway. The hypothesis that BD bears a considerable potential in further research on its dual therapeutic effect on hypertensive patients with poor skeletal conditions was proved within the limitations of the present study.