High-throughput sequencing identifies salivary microbiota in Chinese caries-free preschool children with primary dentition.
- Author:
Lei XU
1
;
Zhifang WU
1
;
Yuan WANG
1
;
Sa WANG
1
;
Chang SHU
1
;
Zhuhui DUAN
2
;
Shuli DENG
3
Author Information
- Publication Type:Journal Article
- Keywords: 16S rDNA V3-V4 hypervariable regions; Caries-free; Illumina MiSeq; Preschool children; Primary dentition; Salivary microbiota
- From: Journal of Zhejiang University. Science. B 2021;22(4):285-294
- CountryChina
- Language:English
-
Abstract:
OBJECTIVES:The study aimed at identifying salivary microbiota in caries-free Chinese preschool children using high-throughput sequencing.
METHODS:Saliva samples were obtained from 35 caries-free preschool children (18 boys and 17 girls) with primary dentition, and 16S ribosomal DNA (rDNA) V3-V4 hypervariable regions of the microorganisms were analyzed using Illumina MiSeq.
RESULTS:At 97% similarity level, all of these reads were clustered into 334 operational taxonomic units (OTUs). Among these, five phyla (Firmicutes, Proteobacteria, Actinobacteria, Bacteroidetes, and Candidate division TM7) and 13 genera (
CONCLUSIONS:Our results revealed the diversity and composition of salivary microbiota in caries-free preschool children, with little difference between male and female subjects. Identity of the core microbiome, coupled with prediction of gene function, deepens our understanding of oral microbiota in caries-free populations and provides basic information for associating salivary microecology and oral health.