High-throughput sequencing identifies salivary microbiota in Chinese caries-free preschool children with primary dentition.

Lei XU; Zhifang WU; Yuan Wang; Sa Wang; Chang Shu; Zhuhui Duan; Shuli Deng

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High-throughput sequencing identifies salivary microbiota in Chinese caries-free preschool children with primary dentition.

Author: Lei XU ¹ ; Zhifang WU ¹ ; Yuan WANG ¹ ; Sa WANG ¹ ; Chang SHU ¹ ; Zhuhui DUAN ² ; Shuli DENG ³
Author Information

1. The Affiliated Hospital of Stomatology, School of Stomatology, Zhejiang University School of Medicine, and Key Laboratory of Oral Biomedical Research of Zhejiang Province, Hangzhou 310006, China.
2. Department of Stomatology, Luoyang Central Hospital Affiliated to Zhengzhou University, Luoyang 471009, China.
3. The Affiliated Hospital of Stomatology, School of Stomatology, Zhejiang University School of Medicine, and Key Laboratory of Oral Biomedical Research of Zhejiang Province, Hangzhou 310006, China. dengshuli@zju.edu.cn.
Publication Type:Journal Article
Keywords: 16S rDNA V3-V4 hypervariable regions; Caries-free; Illumina MiSeq; Preschool children; Primary dentition; Salivary microbiota
From: Journal of Zhejiang University. Science. B 2021;22(4):285-294
CountryChina
Language:English
Abstract: OBJECTIVES:The study aimed at identifying salivary microbiota in caries-free Chinese preschool children using high-throughput sequencing.
METHODS:Saliva samples were obtained from 35 caries-free preschool children (18 boys and 17 girls) with primary dentition, and 16S ribosomal DNA (rDNA) V3-V4 hypervariable regions of the microorganisms were analyzed using Illumina MiSeq.
RESULTS:At 97% similarity level, all of these reads were clustered into 334 operational taxonomic units (OTUs). Among these, five phyla (Firmicutes, Proteobacteria, Actinobacteria, Bacteroidetes, and Candidate division TM7) and 13 genera (
CONCLUSIONS:Our results revealed the diversity and composition of salivary microbiota in caries-free preschool children, with little difference between male and female subjects. Identity of the core microbiome, coupled with prediction of gene function, deepens our understanding of oral microbiota in caries-free populations and provides basic information for associating salivary microecology and oral health.