Evaluation and application of an efficient plant DNA extraction protocol for laboratory and field testing.

Qi Wang; Xiaoxia Shen; Tian Qiu; Wei WU; Lin LI; Zhi'an Wang; Huixia Shou

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Evaluation and application of an efficient plant DNA extraction protocol for laboratory and field testing.

Author: Qi WANG ¹ ; Xiaoxia SHEN ² ; Tian QIU ¹ ; Wei WU ³ ; Lin LI ¹ ; Zhi'an WANG ² ; Huixia SHOU ⁴
Author Information

1. State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou 310058, China.
2. Zhejiang Institute of Chinese Medicine, Hangzhou 310023, China.
3. Shanghai YouLong Biotech Co., Ltd., Shanghai 200063, China.
4. State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou 310058, China. huixia@zju.edu.cn.
Publication Type:Journal Article
Keywords: Cellulose filter paper; Chinese medicine ultra-fine powder; DNA extraction; Field test; Genetically modified (GM) crops; Rapid molecular identification
From: Journal of Zhejiang University. Science. B 2021;22(2):99-111
CountryChina
Language:English
Abstract: Nucleic acids in plant tissue lysates can be captured quickly by a cellulose filter paper and prepared for amplification after a quick purification. In this study, a published filter paper strip method was modified by sticking the filter paper on a polyvinyl chloride resin (PVC) sheet. This modified method is named EZ-D, for EASY DNA extraction. Compared with the original cetyl trimethylammonium bromide (CTAB) method, DNA extracted by EZ-D is more efficient in polymerase chain reaction (PCR) amplification due to the more stable performance of the EZ-D stick. The EZ-D method is also faster, easier, and cheaper. PCR analyses showed that DNA extracted from several types of plant tissues by EZ-D was appropriate for specific identification of biological samples. A regular PCR reaction can detect the EZ-D-extracted DNA template at concentration as low as 0.1 ng/μL. Evaluation of the EZ-D showed that DNA extracts could be successfully amplified by PCR reaction for DNA fragments up to 3000 bp in length and up to 80% in GC content. EZ-D was successfully used for DNA extraction from a variety of plant species and plant tissues. Moreover, when EZ-D was combined with the loop-mediated isothermal amplification (LAMP) method, DNA identification of biological samples could be achieved without the need for specialized equipment. As an optimized DNA purification method, EZ-D shows great advantages in application and can be used widely in laboratories where equipment is limited and rapid results are required.