Effect of advanced glycation end products on autophagic ability in osteoblasts.
10.11817/j.issn.1672-7347.2021.190401
- Author:
Dan LUO
1
;
Yun HU
2
;
Yu TANG
3
;
Xiaoqian DING
3
;
Caiyu LI
2
;
Leilei ZHENG
4
Author Information
1. Department of Orthodontics, Stomatological Hospital of Chongqing Medical University, Chongqing 401145. 2017110912@stu.cqmu.edu.cn.
2. Chongqing Key Laboratory of Oral Diseases and Biomedicine Science, Chongqing 401145.
3. Department of Orthodontics, Stomatological Hospital of Chongqing Medical University, Chongqing 401145.
4. Department of Orthodontics, Stomatological Hospital of Chongqing Medical University, Chongqing 401145. zhengleileicqmu@hospital.cqmu.edu.cn.
- Publication Type:Journal Article
- Keywords:
Beclin1;
LC3;
advanced glycation end products;
autophagy;
osteogenic differentiation
- MeSH:
Animals;
Autophagy;
Bone Marrow Cells;
Cell Differentiation;
Cells, Cultured;
Glycation End Products, Advanced/pharmacology*;
Humans;
Osteoblasts;
Osteogenesis;
Rats;
Rats, Sprague-Dawley
- From:
Journal of Central South University(Medical Sciences)
2021;46(4):361-367
- CountryChina
- Language:English
-
Abstract:
OBJECTIVES:Excessive production of AGEs in diabetic patients will affect the normal function of osteoblasts, and this process may be related to autophagy of osteoblasts. This study aims to explore the effect of advanced glycation end products (AGEs) on autophagic activity during osteogenic differentiation in rat bone marrow mesenchymal stem cells (BMSCs).
METHODS:BMSCs were isolated and cultured in vitro, treated with different concentrations (0, 50, 100, 200, and 400 mg/L) of AGEs for different time (3, 6, 12, 24, 48, and 72 h). The proliferation activity was detected by CCK-8 method. The mRNA and protein expression levels of Beclin1 and LC3 in cells were detected by real-time PCR and Western blotting, respectively.The autophagic vacuoles were observed under the transmission electron microscope. The cells were treated with autophagy promoter rapamycin or autophagy inhibitor 3MA. After 7 days of osteogenic induction, we performed alkaline phosphatase (ALP) staining and real-time PCR to detect the mRNA expression levels of osteogenesis-related genes.
RESULTS:In the low-concentration groups, the proliferation activity in BMSCs was increased (
CONCLUSIONS:Low concentration of AGEs can enhance the proliferative activity of BMSCs and promote osteogenic differentiation by accelerating autophagy. High concentration of AGEs can suppress the proliferation of BMSCs and inhibit osteogenic differentiation by reducing autophagy.
