Genetic polymorphism of 23 autosomal STR loci in Han population from Yuncheng, Shanxi Province.
10.11817/j.issn.1672-7347.2021.190357
- Author:
Hongyan GAO
1
,
2
;
Jian YU
3
;
Xiaodan FENG
4
;
Xiaohong WU
5
;
Li LUO
1
,
6
;
Xianfeng LI
7
;
Chao LIU
7
;
Pengyu CHEN
1
,
8
Author Information
1. Key Laboratory of Cell Engineering in Guizhou Province
2. Affiliated Hospital of Zunyi Medical University, Zunyi Guizhou 563099. 1024097921@qq.com.
3. Center of Forensic Expertise, Affiliated Hospital of Zunyi Medical University, Zunyi Guizhou 563099.
4. Wuhou Subbureau, Chengdu Public Security Bureau, Chengdu 610041.
5. Meitan Public Security Bureau, Zunyi Guizhou 563003.
6. Affiliated Hospital of Zunyi Medical University, Zunyi Guizhou 563099.
7. Department of Medical Genetics, Zunyi Medical University, Zunyi Guizhou 563099, China.
8. Affiliated Hospital of Zunyi Medical University, Zunyi Guizhou 563099. pychenfs@163.com.
- Publication Type:Journal Article
- Keywords:
AGCU EX25;
Shanxi Yuncheng Han nationality;
genetic polymorphism;
population genetic;
short tandem repeat
- MeSH:
Asian Continental Ancestry Group/genetics*;
China;
Ethnic Groups/genetics*;
Gene Frequency;
Genetic Loci;
Genetics, Population;
Humans;
Microsatellite Repeats/genetics*;
Polymorphism, Genetic
- From:
Journal of Central South University(Medical Sciences)
2021;46(4):351-360
- CountryChina
- Language:English
-
Abstract:
OBJECTIVES:Due to the genetic feature of high diversity than other DNA markers, short tandem repeat (STR) plays key roles in forensic, anthropology, and population genetics. Newly introduced multiple STR kit is more valuable because of the greatly improved discriminatory power with the increase in the number of STR loci. The genetic polymorphic data are essential for the application and research in specific population. This study aims to investigate the genetic polymorphism of Han population residing in Yuncheng district, Shanxi Province, to evaluate the application of 23 STR loci in forensic personal identification and paternity test, and to explore the genetic relationship of Han population between Yuncheng and other populations.
METHODS:A total of 23 STR loci were amplified from 525 healthy unrelated individuals from the Han nationality in Yuncheng, Shanxi Province using the AGCU EX25 amplification kit. The products were detected and separated by ABI 3500 Genetic Analyzer. Alleles were genotyped by GeneMapper ID (Version 3.2) software, and corresponding frequencies and forensic parameters were calculated. We calculated the genetic distance and plotted the neighboring-joining tree with other 13 population.
RESULTS:The allele frequency of the 23 STRs ranged from 0.0010 to 0.5090. No deviation from Hardy-Weinberg equilibrium (
CONCLUSIONS:These 23 STRs are highly genetic polymorphic and informative in the Han population of Yuncheng, Shanxi Province, which can provide basic data for forensic personal identification, paternity testing, and population genetic research.
