Transcriptome analysis of mild hypothermia protection against radiation
10.11817/j.issn.1672-7347.2021.190727
- Author:
Yuanfei WANG
1
,
2
,
3
;
Xingang WANG
1
,
2
,
4
;
Wenli XU
1
,
2
,
4
;
Yongli WANG
1
,
2
,
4
;
Liqun HUANG
1
,
2
,
4
;
Juan YUE
1
,
2
,
4
;
Quan AN
1
,
2
,
4
;
Shufang LI
1
,
2
,
5
Author Information
1. Drug Safety Evaluation Center of China Institute for Radiation Protection
2. Shanxi Key Laboratory of Drug Toxicology and Drug for Radiation Injury
3. Key Laboratory on Radiotoxicology and Radiopharmaceutical Preclinical Evaluation, China National Nuclear Corporation, Taiyuan 030006, China. wangyuanfei66@yeah.net.
4. Key Laboratory on Radiotoxicology and Radiopharmaceutical Preclinical Evaluation, China National Nuclear Corporation, Taiyuan 030006, China.
5. Key Laboratory on Radiotoxicology and Radiopharmaceutical Preclinical Evaluation, China National Nuclear Corporation, Taiyuan 030006, China. lishufang821@163.com.
- Publication Type:Journal Article
- Keywords:
RNA-sequence;
mild hypothermia;
radiation-induced lung injury;
tight junction pathway
- MeSH:
Animals;
Gene Expression Profiling;
Hypothermia;
Lung Injury;
Male;
RNA-Seq;
Rats;
Rats, Sprague-Dawley;
Transcriptome
- From:
Journal of Central South University(Medical Sciences)
2021;46(4):345-350
- CountryChina
- Language:English
-
Abstract:
OBJECTIVES:To analyze the differentially expressed genes (DEGs) with radiation-induced rat lung injury, and to reveal the protective mechanism for mild hypothermia in the radiation-induced lung injury in rats at the transcriptome level.
METHODS:A total of 10 male SD rats aged 6-8 weeks were randomly divided into 2 groups to establish a rat model of radiation-induced lung injury, and one group was treated with mild hypothermia. RNA was extracted from left lung tissue of each group, and sequenced by BGISEQ-500 platform. Significance analysis of DEGs was carried out by edgeR software. Gene ontology (GO) function enrichment analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis were used to analyze the gene function. Then 5 key DEGs were verified by real-time reverse transcription PCR (real-time RT-PCR).
RESULTS:There were 2 790 DEGs (false discovery rate<0.001, |log
CONCLUSIONS:The DEGs and pathways related to mild hypothermia protection against radiation-induced lung injury in rats are obtained, which provides an experimental basis for the protection of mild hypothermia against radiation-induced lung injury.
