Effect of autophagy on oxalate-induced toxicity of human proximal renal tubular epithelial cell.
10.11817/j.issn.1672-7347.2021.190299
- Author:
Danqi LIU
1
;
Jun LEI
2
;
Kai HUANG
2
;
Guangming YIN
3
Author Information
1. Department of Urology, Third Xiangya Hospital, Central South University, Changsha 410013, China. ldqmailbox@163.com.
2. Department of Urology, Third Xiangya Hospital, Central South University, Changsha 410013, China.
3. Department of Urology, Third Xiangya Hospital, Central South University, Changsha 410013, China. yingm75@126.com.
- Publication Type:Journal Article
- Keywords:
apoptosis;
autophagy;
injury;
oxalate;
renal tubules
- MeSH:
Apoptosis;
Autophagy;
Cell Line;
Epithelial Cells;
Humans;
Oxalates/toxicity*
- From:
Journal of Central South University(Medical Sciences)
2021;46(3):221-226
- CountryChina
- Language:English
-
Abstract:
OBJECTIVES:To investigate the role of autophagy in oxalate-induced toxicity of human proximal renal tubular epithelial cell (HK-2).
METHODS:HK-2 cells were exposed to oxalate (1 mmol/L) for 2 h and 3-methyladenine (3-MA) was used to inhibit autophagy. Then Western blotting was used to measure the expression of autophagy-related protein LC3II. Cell viability and cell apoptosis were measured by MTT assay and flow cytometry assay, respectively.
RESULTS:Cytoplasmic vacuolization was observed in HK-2 cells after treating with oxalate for 2 h. However, 3-MA showed no effects on the formation of cytoplasmic vacuolization regardless of the dose at 1 or 5 mmol/L. The expression of LC3II protein was significantly increased in the HK-2 cells in the presence of oxalate (0.62±0.03 vs 0.35±0.02,
CONCLUSIONS:Autophagy of HK-2 cells is enhanced by oxalate at the concentration of 1 mmol/L. Inhibition of 3-MA-induced autophagy protects HK-2 cells from the oxalate-induced cytotoxicity.
