Oligomycin A promotes radioresistance in HT29 colorectal cancer cells and its mechanisms.
10.11817/j.issn.1672-7347.2021.200063
- Author:
Xiaofei LI
1
;
Ruifang TIAN
2
;
Lihui WANG
2
;
Cong XU
2
;
Hui WU
2
;
Lan LIU
2
;
Chenghui HUANG
3
Author Information
1. Department of Oncology, Third Xiangya Hospital, Central South University, Changsha 410013, China. 1130144700@qq.com.
2. Department of Oncology, Third Xiangya Hospital, Central South University, Changsha 410013, China.
3. Department of Oncology, Third Xiangya Hospital, Central South University, Changsha 410013, China. 86801158@qq.com.
- Publication Type:Journal Article
- Keywords:
colorectal cancer;
glycolysis;
oligomycin A;
phosphofructokinase 1;
radiosensitivity
- MeSH:
Cell Line, Tumor;
Colorectal Neoplasms/genetics*;
HT29 Cells;
Humans;
Oligomycins/pharmacology*;
Radiation Tolerance
- From:
Journal of Central South University(Medical Sciences)
2021;46(2):113-120
- CountryChina
- Language:English
-
Abstract:
OBJECTIVES:Radiotherapy is one of the main therapies for colorectal cancer, but radioresistance often leads to radiotherapy failure. To improve the radioresistance, we explore the effect of oligomycin A, the H
METHODS:The effects of different concentrations of oligomycin A on the survival rate and glycolysis of HT29 colorectal cancer cells at different time points were investigated via MTT and glycolysis assay. siRNA-PFK1 was synthesized in vitro and transfected into HT29 cells. The effects of oligomycin A on radiosensitivity of HT29 colorectal cancer cells were measured via MTT and colony formation assay. Western blotting was used to detect the effect of oligomycin A on the expression of glycolytic enzyme PFK1. We compared difference between the effects of siRNA-PFK1 group and oligomycin A combined with siRNA-PFK1 group on cell survival and glycolysis. After 4 Gy X-ray irradiation, the effects of cell survival and glycolysis between the siRNA-PFK1 group and the oligomycin A combined with siRNA-PFK1 group were compared.
RESULTS:Compared with the 0 μmol/L oligomycin A group, the cell survival rate of HT29 cells treated with 4 μmol/L oligomycin A was significantly increased (
CONCLUSIONS:Oligomycin A can promote the radioresistance of HT29 colorectal cancer cells, which may be related to up-regulation of the PFK1 expression and increase of cell glycolysis.
