Effect of etomidate on the reuniens neuronal activity.
10.11817/j.issn.1672-7347.2021.190576
- Author:
Yulong WANG
1
;
Tingting XUE
2
;
Yongquan CHEN
3
Author Information
1. Department of Anesthesiology, First Affiliated Hospitalof Wannan Medical College, Wuhu Anhui 241000, China. dr_wangyulong@163.com.
2. Department of Anesthesiology, First Affiliated Hospitalof Wannan Medical College, Wuhu Anhui 241000, China.
3. Department of Anesthesiology, First Affiliated Hospitalof Wannan Medical College, Wuhu Anhui 241000, China. chenyq263@163.com.
- Publication Type:Journal Article
- Keywords:
etomidate;
gamma-aminobutyric acid Type A;
patch clamp;
reuniens nucleus
- MeSH:
Animals;
Etomidate/pharmacology*;
Mice;
Mice, Inbred C57BL;
Neurons;
Patch-Clamp Techniques;
Receptors, GABA-A
- From:
Journal of Central South University(Medical Sciences)
2021;46(1):39-46
- CountryChina
- Language:English
-
Abstract:
OBJECTIVES:To explore the effect of etomidate on the neuronal activity of ventral thalamic reuniens nucleus and the underlying mechanisms.
METHODS:Whole-cell patch clamp method was used to explore the effect of etomidate on the activity of ventral thalamic reuniens neurons in the acute brain slices obtained from 4-5 weeks old C57BL/6J mice. The electrophysiological characteristics of ventral thalamic reuniens neurons were recorded in the current clamp mode, and then the effects of etomidate (0.5, 2.0, 8.0 μmol/L etomidate groups) and intralipid (intralipid group) on the discharge frequency and membrane potential of ventral thalamic reuniens neurons were recorded. During the experiment, the ventral thalamic reuniens neuron firing rates (RNFRs) were recorded as F
RESULTS:In the intralipid group, there was no significant difference among the F
CONCLUSIONS:Etomidate can inhibit the activity of ventral thalamic reuniens neurons in concentration-dependent manner, and which is reversible. Etomidate with sub-anesthetic concentration inhibits the activity of ventral thalamic reuniens neurons via targeting the GABA
