Mangiferin attenuates IL
10.11817/j.issn.1672-7347.2021.190287
- Author:
Peng ZHU
1
;
Cong LIU
2
;
Bo LI
2
;
Chen ZHAO
2
;
Tao ZHOU
2
;
Xin XUE
2
;
Bing ZHANG
3
Author Information
1. Department of Orthopedics, Second Affiliated Hospital of Xi'an Medical University, Xi'an 710038, China. pengzhu_374@163.com.
2. Department of Orthopedics, Second Affiliated Hospital of Xi'an Medical University, Xi'an 710038, China.
3. Department of Orthopedics, Second Affiliated Hospital of Xi'an Medical University, Xi'an 710038, China. 461558159@qq.com.
- Publication Type:Journal Article
- Keywords:
IL-1β;
apoptosis;
chondrocyte;
mangiferin;
osteoarthritis;
phosphoinositide 3-kinase/Akt
- MeSH:
Animals;
Apoptosis;
Chondrocytes;
Interleukin-1beta;
Mice;
Phosphatidylinositol 3-Kinases;
Proto-Oncogene Proteins c-akt;
Xanthones
- From:
Journal of Central South University(Medical Sciences)
2021;46(1):25-31
- CountryChina
- Language:English
-
Abstract:
OBJECTIVES:Chondrocyte apoptosis is an important process in the pathogenesis of osteoarthritis. Mangiferin exerts multiple pharmacological effects such as anti-inflammatory and anti-apoptosis. However, the role of mangiferin in chondrocyte apoptosis is not clear. In this study, we aimed to explore the role of mangiferin in IL-1β-induced chondrocyte apoptosis.
METHODS:ATDC5 cells were randomly divided into a control group, a IL-1β group, a MFN-L group, a MFN-M group, a MFN-H group and a MFN+LY294002 group. Cells in the control group were treated with IL-1β (10 ng/mL) for 24 h; cells in the MFN-L group, the MFN-M group and the MFN-H group were pretreated with 5, 10 and 20 μmol/L mangiferin for 1 h respectively, and then they were treated with IL-1β (10 ng/mL) for 24 h; cells in the MFN+LY294002 group were treated with LY294002 (25 μmol/L) for 1 h, then mangiferin (20 μmol/L) and IL-1β (10 ng/mL) for 1 h and 24 h, respectively. Cell viability was detected by CCK-8 assay and cell apoptosis was measured by flow cytometry. Colorimetric assay was conducted to measure the caspase-3 activity. The protein levels of Bcl-2, Bax, and phosphoinositide 3-kinase (PI3K)/Akt signaling pathway related proteins were detected by Western blotting.
RESULTS:Compared to the control group, cell viability was significantly decreased; cell apoptosis, caspase-3 activity and Bax protein expression were significantly increased; the protein levels of Bcl-2, p-PI3K, and p-Akt were significantly decreased in the IL-1β group (all
CONCLUSIONS:Mangiferin could attenuate IL-1β-induced apoptosis of the mice chondrocytes, which is mediated by the activation of PI3K/Akt signaling pathway.
