Mechanisms for propofol in inhibiting the proliferation and invasion of glioma U87 cells and its effect on miR-134 expression.
10.11817/j.issn.1672-7347.2021.190734
- Author:
Fengdan LI
1
;
Hua ZHANG
2
;
Fuchao WANG
2
;
Yingxia ZHENG
2
Author Information
1. Department of Anesthesiology, Hengshui People's Hospital, Hengshui Hebei 053000, China. lidanfeng895@163.com.
2. Department of Anesthesiology, Hengshui People's Hospital, Hengshui Hebei 053000, China.
- Publication Type:Journal Article
- Keywords:
glioma cell U87;
invasion;
miR-134;
proliferation;
propofol
- MeSH:
Cell Line, Tumor;
Cell Movement;
Cell Proliferation;
Glioma/genetics*;
Humans;
Matrix Metalloproteinase 2/genetics*;
MicroRNAs/genetics*;
Phosphatidylinositol 3-Kinases/genetics*;
Propofol/pharmacology*;
Proto-Oncogene Proteins c-akt/genetics*
- From:
Journal of Central South University(Medical Sciences)
2021;46(1):18-24
- CountryChina
- Language:English
-
Abstract:
OBJECTIVES:To investigate the effects of propofol on the proliferation and invasion of glioma U87 cells and to explore the possible anti-tumor mechanisms.
METHODS:The glioma U87 cells was divided into a blank group, a positive control group, and the propofol groups (1.00, 2.00 or 5.00 mmol/L). Cell counting kit-8 (CCK-8) was used to detect cell proliferation; Transwell method was used to detect the effect of propofol on invasion and migration of U87 cells; real-time PCR was used to detect the expression of microRNA-134 (miR-134); Western blotting was used to detect the expression levels of reproduction-related protein Ki-67, invasion-related protein metalloproteinase-2 (MMP-2), metalloproteinase-9 (MMP-9) and phosphatidylinositol 3 kinase (PI3K)/protein kinase B (Akt) signaling pathway-related protein.
RESULTS:Compared with the blank group, the proliferation, invasion and migration capacity of U87 cells were reduced in the positive control group and the propofol groups after 48 hours (all
CONCLUSIONS:Propofol can decrease the proliferation rate, and the invasion and migration abilities of U87 cells, which may be achieved by up-regulation of miR-134 and suppression of PI3K/Akt signaling pathway.
