Development of the Enzyme Immunoassay for the Detection of Anti-HSV-2 Antibody with HSV-2 Specific Monoclonal Antibody.
- Author:
Chung Gyu PARK
1
;
Jae Won PARK
;
Dae Joong KIM
;
Jinhee KIM
;
Eung Soo HWANG
;
Hyun Moo LEE
;
Ai Young LEE
;
Chang Yong CHA
Author Information
1. Department of Microbiology, Seoul National University College of Medicine and Institute of Endemic Diseases, Seoul National University Medical Research Center.
- Publication Type:Original Article
- Keywords:
Herpes simplex virus type 2 (HSV- 2);
Monoclonal antibody;
Immunofluorescent staining;
Competitive ELISA
- MeSH:
Antibodies;
Blotting, Western;
Diagnosis;
Enzyme-Linked Immunosorbent Assay;
Herpes Genitalis;
Herpesvirus 1, Human;
Herpesvirus 2, Human*;
Humans;
Immunoenzyme Techniques*;
Korea;
Mass Screening;
Prevalence
- From:Korean Journal of Infectious Diseases
1999;31(4):309-316
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: The serological diagnosis of herpes simplex virus type 2 (HSV-2) infection has pitfalls, in that most of the antibodies against HSV-2 cross-react with HSV-1 and the prevalence of HSV-1 infection is high, especially in Korea. In this study, we tried to establish the serological diagnostic method, which could detect and measure the specific antibodies against HSV- 2 by competitive immunofluorescent staining method as well as competitive ELISA based on the specific monoclonal antibody, MH2-7. METHODS: Immunofluorescent staining and western blot analysis were used to characterize the antigens recognized by MH2-7. Competitive immunofluorescent staining (IF), competitive enzyme immunoassay (ELISA), and western blot analysis were used to detect specific antibodies against HSV-2 in patients' sera. RESULTS: In western blot analysis, the sera from two of six patients clinically diagnosed as genital herpes showed characteristic band patterns, which have been known to be compatible with HSV-2 infection. In competitive immunofluorescent staining, only the sera from the two patients clinically diagnosed as genital herpes and with characteristic band pattern showed competition with MH2-7 monoclonal antibody. The dilution range of the serum showing specific competition was between 1:10 and 1:80. Competitive ELISA was also performed and evaluated as the diagnostic efficacy as ELISA has been known to be advantageous over IF staining in mass screening. The result showed linear dose-response relationship for the patient's sera in inhibition of the reactivity of MH2-7. CONCLUSION: We suggest that the competitive immunofluorescent staining method and competitive ELISA based on the specific monoclonal antibody MH2-7 is a simple, accurate, and precise method, which can be used in serological diagnosis of HSV-2 infection.
