Ginsenoside Rb1 Protects Human Umbilical Vein Endothelial Cells against High Glucose-Induced Mitochondria-Related Apoptosis through Activating SIRT3 Signalling Pathway.
10.1007/s11655-020-3478-8
- Author:
Shi-Ye KE
1
;
Shu-Jie YU
1
;
Ding-Hui LIU
1
;
Guang-Yao SHI
1
;
Min WANG
1
;
Bin ZHOU
1
;
Lin WU
1
;
Zhi-Ming SONG
2
;
Jie-Ming ZHU
1
;
Chao-Dong WU
3
;
Xiao-Xian QIAN
4
Author Information
1. Department of Cardiology, the Third Affiliated Hospital of Sun Yat-Sen University, Guangzhou, 510630, China.
2. Department of Cardiology, the First Affiliated Hospital of Henan University, Kaifeng, Henan Province, 475001, China.
3. Department of Nutrition and Food Science, Texas A&M University, College Station, TX, 77843, United States of America.
4. Department of Cardiology, the Third Affiliated Hospital of Sun Yat-Sen University, Guangzhou, 510630, China. qianxx@mail.sysu.edu.cn.
- Publication Type:Journal Article
- Keywords:
SIRT3 signalling pathway;
apoptosis;
ginsenoside Rb1;
high glucose;
human umbilical vein endothelial cells;
mitochondria
- From:
Chinese journal of integrative medicine
2021;27(5):336-344
- CountryChina
- Language:English
-
Abstract:
OBJECTIVE:To investigate whether ginsenoside Rb1 (Rb1) can protect human umbilical vein endothelial cells (HUVECs) against high glucose-induced apoptosis and examine the underlying mechanism.
METHODS:HUVECs were divided into 5 groups: control group (5.5 mmol/L glucose), high glucose (HG, 40 mmol/L) treatment group, Rb1 (50 µ mol/L) treatment group, Rb1 plus HG treatment group, and Rb1 and 3-(
RESULTS:Rb1 ameliorated survival in cells in which apoptosis was induced by high glucose (P<0.05 or P<0.01). Upon the addition of Rb1, mitochondrial and intracellular reactive oxygen species generation and malondialdehyde levels were decreased (P<0.01), while the activities of antioxidant enzymes were increased (P<0.05 or P<0.01). Rb1 preserved the mitochondrial membrane potential and reduced the release of Cyt-c from the mitochondria into the cytosol (P<0.01). In addition, Rb1 upregulated mitochondrial biogenesis-associated proteins (P<0.01). Notably, the cytoprotective effects of Rb1 were correlated with SIRT3 signalling pathway activation (P<0.01). The effect of Rb1 against high glucose-induced mitochondria-related apoptosis was restrained by 3-TYP (P<0.05 or P<0.01).
CONCLUSION:Rb1 could protect HUVECs from high glucose-induced apoptosis by promoting mitochondrial function and suppressing oxidative stress through the SIRT3 signalling pathway.
