Edible Bird's Nest Attenuates Menopause-Related Bone Degeneration in Rats via Increaing Bone Estrogen-Receptor Expression.
10.1007/s11655-019-3209-1
- Author:
Zhi-Ping HOU
1
;
Shi-Ying TANG
2
;
Hai-Ru JI
2
;
Pei-Yuan HE
3
;
Yu-Hong LI
2
;
Xian-Ling DONG
2
;
Meng-Nan DU
2
;
Ismail MAZNAH
4
;
Wen-Jing HE
5
Author Information
1. Department of Pathology, Chengde Medical University, Chengde, Hebei Province, 067000, China. zhipinghou@163.com.
2. Department of Pathology, Chengde Medical University, Chengde, Hebei Province, 067000, China.
3. Department of Gastroenterology, The Affiliated Hospital of Chengde Medical University, Chengde, Hebei Province, 067000, China.
4. Laboratory of Molecular Biomedicine, Institute of Bioscience, Universiti Putra Malaysia, Serdang, Selangor, 43400, Malaysia.
5. Department of Gastroscopy, Kuancheng Manchu Autonomous County Hospital, Chengde, Hebei Province, 067000, China.
- Publication Type:Journal Article
- Keywords:
bone;
edible bird’s nest;
menopause;
osteoporosis;
ovariectomy;
rats
- From:
Chinese journal of integrative medicine
2021;27(4):280-285
- CountryChina
- Language:English
-
Abstract:
OBJECTIVE:To investigate the mechanistic basis for the attenuation of bone degeneration by edible bird's nest (EBN) in ovariectomized rats.
METHODS:Forty-two female Sprage-Dawley rats were randomized into 7 groups (6 in each group). The ovariectomized (OVX) and OVX + 6%, 3%, and 1.5% EBN and OVX +estrogen groups were given standard rat chow alone, standard rat chow +6%, 3%, and 1.5% EBN, or standard rat chow +estrogen therapy (0.2mg/kg per day), respectively. The sham-operation group was surgically opened without removing the ovaries. The control group did not have any surgical intervention. After 12 weeks of intervention, blood samples were taken for serum estrogen, osteocalcin, and osteoprotegerin, as well as the measurement of magnesium, calcium abd zinc concentrations. While femurs were removed from the surrounding muscles to measure bone mass density using the X-ray edge detection technique, then collected for histology and estrogen receptor (ER) immunohistochemistry.
RESULTS:Ovariectomy altered serum estrogen levels resulting in increased food intake and weight gain, while estrogen and EBN supplementation attenuated these changes. Ovariectomy also reduced bone ER expression and density, and the production of osteopcalcin and osteorotegerin, which are important pro-osteoplastic hormones that promote bone mineraliztion and density. Conversely, estrogen and EBN increased serum estrogen levels leading to increased bone ER expression, pro-osteoplastic hormone production and bone density (all P<0.05).
CONCLUSION:EBN could be used as a safe alternative to hormone replacement therapys for managing menopausal complications like bone degeneration.
