Effect of IL-15 addition on asbestos-induced suppression of human cytotoxic T lymphocyte induction.
10.1186/s12199-021-00967-9
- Author:
Naoko KUMAGAI-TAKEI
1
;
Yasumitsu NISHIMURA
2
;
Hidenori MATSUZAKI
1
;
Suni LEE
1
;
Kei YOSHITOME
1
;
Tatsuo ITO
1
;
Takemi OTSUKI
1
Author Information
1. Department of Hygiene, Kawasaki Medical School, Kurashiki, 701-0192, Japan.
2. Department of Hygiene, Kawasaki Medical School, Kurashiki, 701-0192, Japan. yas@med.kawasaki-m.ac.jp.
- Publication Type:Journal Article
- Keywords:
Asbestos;
CTL;
Granzyme B;
IL-15;
Proliferation
- MeSH:
Asbestos/adverse effects*;
CD8-Positive T-Lymphocytes/metabolism*;
Humans;
Interleukin-15/pharmacology*;
Lymphocyte Activation/immunology*;
T-Lymphocytes, Cytotoxic/metabolism*
- From:Environmental Health and Preventive Medicine
2021;26(1):50-50
- CountryJapan
- Language:English
-
Abstract:
BACKGROUND:Asbestos fibers possess tumorigenicity and are thought to cause mesothelioma. We have previously reported that exposure to asbestos fibers causes a reduction in antitumor immunity. Asbestos exposure in the mixed lymphocyte reaction (MLR) showed suppressed induction of cytotoxic T lymphocytes (CTLs), accompanied by a decrease in proliferation of CD8
METHODS:For MLR, human peripheral blood mononuclear cells (PBMCs) were cultured with irradiated allogenic PBMCs upon exposure to chrysotile B asbestos at 5 μg/ml for 7 days. After 2 days of culture, IL-15 was added at 1 ng/ml. After 7 days of MLR, PBMCs were collected and analyzed for phenotypic and functional markers of CD8
RESULTS:IL-15 addition partially reversed the decrease in CD3
CONCLUSION:These findings indicate that CTLs induced upon exposure to asbestos possess dysfunctional machinery that can be partly compensated by IL-15 supplementation, and that IL-15 is more effective in the recovery of proliferation and granzyme B levels from asbestos-induced suppression of CTL induction compared with IL-2.
