Optimization and validation of a highly sensitive method for determining glyphosate in human urine by solid-phase extraction and liquid chromatography with tandem mass spectrometry: a methodological study.
10.1186/s12199-020-00918-w
- Author:
Hiroshi NOMURA
1
;
Risa HAMADA
1
;
Isao SAITO
1
;
Kunihiko NAKANE
2
;
Ritsuko SAWA
3
;
Miwa UKAI
3
;
Eiji SHIBATA
4
;
Mitsuo SATO
5
;
Michihiro KAMIJIMA
6
;
Jun UEYAMA
7
Author Information
1. Department of Biomolecular Sciences, Field of Omics Health Sciences, Nagoya University Graduate School of Medicine, 1-1-20 Daiko-minami, Higashi-ku, Nagoya, Aichi, 461-8673, Japan.
2. Okazaki City Public Health Center, 2-1-1 Wakamiya-cho, Okazaki, Aichi, 444-8545, Japan.
3. The Association for Preventive Medicine of Japan, 1-19-10 Mouri, Koto-ku, Tokyo, 135-0001, Japan.
4. Aichi Medical University Health Service, 1-1 Yazakokarimata, Nagakute, Aichi, 480-1195, Japan.
5. Department of Host Defense Sciences, Field of Omics Health Sciences, Nagoya University Graduate School of Medicine, 1-1-20 Daiko-minami, Higashi-ku, Nagoya, Aichi, 461-8673, Japan.
6. Department of Occupational and Environmental Health, Nagoya City University Graduate School of Medical Sciences, 1 Kawasumi, Mizuho-cho, Mizuho-ku, Nagoya, Aichi, 467-8601, Japan.
7. Department of Biomolecular Sciences, Field of Omics Health Sciences, Nagoya University Graduate School of Medicine, 1-1-20 Daiko-minami, Higashi-ku, Nagoya, Aichi, 461-8673, Japan. ueyama@met.nagoya-u.ac.jp.
- Publication Type:Validation Study
- Keywords:
Biomonitoring;
Glyphosate;
Human urine;
LC-MS/MS;
Solid-phase extraction
- MeSH:
Adult;
Aged;
Chromatography, Liquid/methods*;
Female;
Glycine/urine*;
Humans;
Male;
Middle Aged;
Solid Phase Extraction/methods*;
Tandem Mass Spectrometry/methods*
- From:Environmental Health and Preventive Medicine
2020;25(1):83-83
- CountryJapan
- Language:English
-
Abstract:
BACKGROUND:Glyphosate and its salt formulations are nonselective herbicides that have been extensively used worldwide, both for residential and agricultural purposes. The possible carcinogenicity and teratogenicity of glyphosate remain to be elucidated. We developed a sensitive and high-throughput analytical method for urinary glyphosate using liquid chromatography-tandem mass spectrometry with the aim of contributing to glyphosate exposure assessment in epidemiological studies.
METHODS:After urine dilution (creatinine matching dilution to 0.05 g creatinine/L), glyphosate was extracted using two types of solid phase extraction columns (SCX and NH2) with automated sample preparation instruments. The eluate was dried and dissolved in the mobile phase, followed by liquid chromatography-tandem mass spectrometry analysis. The optimized method was applied to urine samples obtained from 54 Japanese adults and children.
RESULTS:The results from the validation study demonstrated good recoveries (91.0-99.6%), within- and between-run precisions (< 15%), low detection limits (0.1 μg/L), and lower limit of quantification (0.3 μg/L). The detection frequency and median concentration of the urinary glyphosate in Japanese subjects were 59% and 0.25 μg/L (0.34 μg/g creatinine).
CONCLUSIONS:Our reliable determination method was successful in measuring urinary glyphosate concentration. Moreover, this is the first biomonitoring report of urinary glyphosate levels in the Japanese general population.
