Correlation of Mycoplasma genitalium infection with semen parameters and sperm DNA integrity in male infertility patients.
- Author:
Qiang FENG
1
;
Zhi-Wei MA
1
;
Yu WANG
1
;
Ming-Xing QIU
1
Author Information
1. Department of Urology, Sichuan Provincial People's Hospital / Sichuan Academy of Medical Sciences, Chengdu, Sichuan 610072, China .
- Publication Type:Journal Article
- Keywords:
Mycoplasma genitalium infection;
male;
semen quality;
sperm DNA fragmentation;
infertility
- MeSH:
DNA Fragmentation;
Humans;
Infertility, Male/microbiology*;
Male;
Mycoplasma Infections/complications*;
Mycoplasma genitalium;
Semen;
Semen Analysis;
Sperm Count;
Sperm Motility;
Spermatozoa
- From:National Journal of Andrology
2020;26(10):900-905
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To analyze the relationship of Mycoplasma genitalium (MG) infection with routine semen parameters and sperm DNA integrity in male infertility patients.
METHODS:Totally, 114 semen samples, 34 MG-positive and 80 MG-negative, were collected from male infertility patients and subjected to routine semen analysis with the computer-assisted sperm analysis system, Papanicolaou staining for observation of sperm morphology, and sperm chromatin diffusion (SCD) test for detection of sperm DNA integrity. Semen parameters and DNA integrity were compared between the MG-positive and MG-negative groups with SPSS 21.0 statistical software and the relationship between the semen parameters and DNA integrity analyzed by Pearson correlation analysis.
RESULTS:The MG-positive samples, compared with the MG-negative ones, showed significantly decreased semen volume (［2.87 ± 0.37］ vs ［3.86 ± 0.43］ ml, P < 0.01), sperm concentration (［29.05 ± 6.17］ vs ［32.56 ± 5.97］ ×10⁶/ml, P < 0.01), and percentages of progressively motile sperm (PMS) (［15.86 ± 2.79］% vs ［23.65 ± 3.47］%, P < 0.01) and morphologically normal sperm (MNS) (［6.35 ± 2.06］% vs ［7.14 ± 1.89］%, P < 0.05), increased proportions of non-halo sperm (［15.02 ± 3.52］% vs ［9.72 ± 2.94］%, P <0.01) and small-halo sperm (［16.37 ± 5.26］% vs ［11.07 ± 1.65］%, P < 0.01) and sperm DNA fragmentation index (DFI) (［31.39 ± 3.16］% vs ［20.79 ± 3.59］%, P < 0.01), and reduced proportion of large-halo sperm (［54.75 ± 8.74］% vs ［64.15 ± 9.76］%, P < 0.01). DFI was negatively correlated with the percentages of PMS (r = －0.516, P < 0.05) and MNS (r = －0.429, P < 0.05) in the MG-positive group, but not correlated with any of the routine semen parameters in the MG-negative patients (P > 0.05).
CONCLUSIONS:MG infection may be an important factor affecting sperm quality in male infertility patients. Active prevention and treatment of MG infection can help prevent male infertility.
