The transcription factor NF-kB1 regulates miR-195 expression in prostate cancer.
- Author:
Chun-Hui LIU
1
;
Ya-Li WANG
1
;
Kai LU
1
;
Lei ZHANG
1
;
Guang-Yuan ZHANG
1
;
Bin XU
1
;
Shu-Qiu CHEN
1
;
Ming CHEN
1
Author Information
1. Department of Urology, Zhongda Hospital of Southeast University, Nanjing, Jiangsu 210009, China .
- Publication Type:Journal Article
- Keywords:
miR-195;
prostate cancer;
NF-kB1
- MeSH:
Cell Line, Tumor;
Gene Expression Regulation, Neoplastic;
Humans;
Male;
MicroRNAs/genetics*;
NF-kappa B p50 Subunit/metabolism*;
Promoter Regions, Genetic;
Prostatic Neoplasms/genetics*;
Transcription Factors/metabolism*
- From:National Journal of Andrology
2020;26(10):875-880
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the regulatory effect of the transcription factor NF-kB1 on the expression of miR-195 in prostate cancer (PCa).
METHODS:We analyzed the possibility of NF-kB1 binding to the miR-195 promoter and the expression of NF-kB1 in PCa using the JASPAR and Oncomine databases, respectively, and determined the expressions of NF-kB1 and miR-195 in PCa cells by real-time quantitative PCR after inhibiting the former by interfering RNA targeting NF-kB1. We detected the activity of the luciferase reporter gene after constructing its gene plasmid in the miR-195 promoter region and having it co-transfected with the NF-kB1 plasmid. Then we analyzed the correlation between the expressions of miR-195 and NF-kB1 in the prostate tissue.
RESULTS:NF-kB1 was overexpressed in PCa. After inhibition of the expression of NF-kB1, that of miR-195 was increased in PC-3 and DU-145 cell lines, with a negative correlation between the NF-kB1 and miR-195 expressions in the PCa tissue. The results of luciferase reporter gene assay showed direct binding of NF-kB1 to the miR-195 promoter zone.
CONCLUSIONS:NF-kB1 regulates the expression of miR-195 in prostate cancer.
