Plasma MiR-181b and MiR-194 As Biomarkers for Acute Graft- Versus-Host Disease and Significance of Their Changes.
10.19746/j.cnki.issn.1009-2137.2021.03.048
- Author:
Yan CHEN
1
;
Hui-Lan DENG
1
;
Dong-Liang KANG
1
;
Yi WANG
1
;
Xiu-Li HONG
1
;
Quan-Yi LU
2
Author Information
1. Department of Hematology, Zhongshan Hospital Affiliated to Xiamen University, Xiamen 361004, Fujian Province, China.
2. Department of Hematology, Zhongshan Hospital Affiliated to Xiamen University, Xiamen 361004, Fujian Province, China,E-mail: quanyilu@hotmail.com.
- Publication Type:Journal Article
- MeSH:
Acute Disease;
Biomarkers;
Graft vs Host Disease;
Hematopoietic Stem Cell Transplantation;
Humans;
MicroRNAs;
Transplantation, Homologous
- From:
Journal of Experimental Hematology
2021;29(3):957-962
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the clinical correlation of expression level changes of miR-181b and miR-194 to the pathogenesis of acute graft-versus-host disease (aGVHD), and determine plasma miR-181b and miR-194 as the potential biomarkers for aGVHD.
METHODS:The plasma samples were collected from 31 patients who underwent allogeneic hematopoietic stem cell transplantation (allo-HSCT) at before HSCT, 15 days after HSCT and onset of aGVHD. The expression levels of miR-181b and miR-194 were detected by quantitative real-time PCR. Receiver-operating characteristic (ROC) curves and the area under the ROC curve (AUC) were used to assess the sensitivity and specificity of miRNA biomarkers for the diagnosis of aGVHD.
RESULTS:MiR-181b and miR-194 downregulated after treatment were significantly upregulated in the plasma at onset of aGVHD (P<0.05), and there was no significant difference in comparison with the level of before HSCT (P>0.05). The expressions of plasma miR-181b and miR-194 collected on day 15 after HSCT were significantly upregulated in the patients with aGVHD in comparison with non-GVHD patients (P<0.05). Moreover, these elevated miRNAs were detected before aGVHD. The AUC of miR-181b predicting aGVHD was 0.91±0.05 (specificity was 0.94, sensitivity was 0.69). The AUC of miR-194 predicting aGVHD was 0.91±0.06 (specificity was 0.94, sensitivity was 0.77).
CONCLUSION:MiR-181b and miR-194 may serve as early biomarkers for the diagnosis and prognosis of aGVHD.
