Regulation of RNA Binding Protein Mbnl1 on Development of Mouse Embryonic Hematopoietic Stem Cells.
10.19746/j.cnki.issn.1009-2137.2021.03.043
- Author:
Ya-Fei XU
1
;
Wan-Bo TANG
2
;
Jie ZHOU
3
;
Bing LIU
4
;
Yu LAN
5
Author Information
1. School of Medicine, Jinan University, Guangzhou 510000, Guangdong Province, China.
2. Academy of Military Medical Sciences, Academy of Military Sciences, Beijing 100850, China.
3. Fifth Medical Center,General Hospital of PLA, Beijing 100071, China.
4. School of Medicine, Jinan University, Guangzhou 510000, Guangdong Province, China,Fifth Medical Center,General Hospital of PLA, Beijing 100071, China,E-mail: bingliu17@yahoo.com.
5. School of Medicine, Jinan University, Guangzhou 510000, Guangdong Province, China,E-mail: rainyblue_1999@126.com.
- Publication Type:Journal Article
- MeSH:
Animals;
DNA-Binding Proteins;
Gonads;
Hematopoiesis;
Hematopoietic Stem Cells;
Mesonephros;
Mice;
RNA-Binding Proteins/genetics*;
Yolk Sac
- From:
Journal of Experimental Hematology
2021;29(3):924-930
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To analyze the dynamic molecular expression characteristics of single cell RNA binding proteins (RBPs) in the development of mouse embryonic hematopoitic stem cells (HSCs), and obtain the functional research target RNA splicing factor--Mbnl1, to clarify the function of Mbnl1 involved in regulating mouse embryonic HSC development.
METHODS:Bioinformatics was used to analyze the single-cell transcriptome data of mouse embryos during HSC development, and the single-cell RBP dynamic molecular expression maps in HSC development was obtained. Mbnl1 was obtained by combining differential analysis and literature research screening. The Mbnl1-knockout mouse model was constructed by the CRISPER/Cas9 technology. Aorta-gonad-mesonephros (AGM) and yolk sac (YS) tissue in two genotype embryos of Mbnl1
RESULTS:The in vitro CFU-C experiment of hematopoietic cells preliminarily indicated that there was no significant difference in the number of cell colonies in AGM region and YS transformed by the two genotypes of Mbnl1
CONCLUSION:Through functional experiments in vivo and in vitro, it has been confirmed that knockout of the RNA splicing factor--Mbnl1 does not affect the development of HSPC in AGM region of mouse embryo.
